Rabbit ILF3 Polyclonal Antibody | anti-ILF3 antibody

ILF3 antibody - C-terminal region

Cat. Num. AAA3201086

• Gene Names: ILF3; CBTF; DRBF; MMP4; MPP4; NF90; NFAR; NF110; NF90a; NF90b; NFAR2; TCP80; DRBP76; NF110b; NFAR-1; TCP110; MPHOSPH4; NF-AT-90
• Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified
• Synonyms: ILF3; Polyclonal Antibody; ILF3 antibody - C-terminal region; anti-ILF3 antibody

• Host: Rabbit
• Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity Purified
• Form/Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Sequence: Synthetic peptide located within the following region: QQSYNQSPYSNYGPPQGKQKGYNHGQGSYSYSNSYNSPGGGGGSDYNYES
• Sequence Length: 894

• Applicable Applications for anti-ILF3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Homology: Cow: 93%; Dog: 100%; Guinea Pig: 86%; Horse: 93%; Human: 100%; Mouse: 79%; Pig: 93%; Rabbit: 86%; Rat: 86%
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ILF3
• Preparation and Storage: For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Rabbit Anti-ILF3 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Testis TissueObserved Staining: Nucleus in spermatogonia and spermatocytesPrimary Antibody Concentration: 1:100Other Working Concentrations: 1:600Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)

Western Blot (WB)

(WB Suggested Anti-ILF3 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Hela cell lysateThere is BioGPS gene expression data showing that ILF3 is expressed in HeLa)

Related Product Information for anti-ILF3 antibody

This is a rabbit polyclonal antibody against ILF3. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: ILF3 may facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. ILF3 can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. ILF3 can regulate protein arginine N-methyltransferase 1 activity. ILF3 may regulate transcription of the IL2 gene during T-cell activation. It can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of interleukin 2. NFAT binds to a sequence in the IL2 enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. The encoded protein, which is primarily localized to ribosomes, probably regulates transcription at the level of mRNA elongation. Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of interleukin 2. NFAT binds to a sequence in the IL2 enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. The encoded protein, which is primarily localized to ribosomes, probably regulates transcription at the level of mRNA elongation. At least three transcript variants encoding three different isoforms have been found for this gene.

Product Categories/Family for anti-ILF3 antibody

Polyclonal; Transcription Factor; RNA Binding Proteins; Cell Cycle Proteins; Transcription Regulation; Chromatin & Nuclear Signaling; Signal Transduction; DNA/RNA/Protein Interactions; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 24234750
• NCBI GeneID: 3609
• NCBI Accession #: NP_036350
• NCBI GenBank Nucleotide #: NM_012218
• UniProt Accession #: Q12906
• Molecular Weight: 95kDa
• NCBI Official Full Name: interleukin enhancer-binding factor 3 isoform a
• NCBI Official Synonym Full Names: interleukin enhancer binding factor 3
• NCBI Official Symbol: ILF3
• NCBI Official Synonym Symbols: CBTF; DRBF; MMP4; MPP4; NF90; NFAR; NF110; NF90a; NF90b; NFAR2; TCP80; DRBP76; NF110b; NFAR-1; TCP110; MPHOSPH4; NF-AT-90
• NCBI Protein Information: interleukin enhancer-binding factor 3
• UniProt Protein Name: Interleukin enhancer-binding factor 3
• Protein Family: Interleukin enhancer-binding factor
• UniProt Gene Name: ILF3
• UniProt Synonym Gene Names: DRBF; MPHOSPH4; NF90; DRBP76; MPP4; NFAR; NF-AT-90; TCP80
• UniProt Entry Name: ILF3_HUMAN

NCBI Description

This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2014]

Uniprot Description

NFAT90: a double-stranded RNA (dsRNA)-binding protein implicated in the regulation of gene expression . Phosphorylated in a double-stranded RNA-dependent manner possibly by PKR. Five different splice-variant isoforms have been described.

Protein type: RNA-binding; Transcription factor; DNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; membrane; mitochondrion; cytoplasm; nucleolus; ribonucleoprotein complex; nucleus

Molecular Function: protein binding; DNA binding; RNA binding; double-stranded RNA binding

Biological Process: transcription, DNA-dependent; negative regulation of viral genome replication; positive regulation of transcription, DNA-dependent; negative regulation of translation; negative regulation of transcription, DNA-dependent; defense response to virus; protein amino acid phosphorylation