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Bovine Myosin regulatory light chain 12B ELISA Kit | MYL12B elisa kit

Bovine Myosin regulatory light chain 12B (MYL12B) ELISA Kit

Gene Names
MYL12B; MRLC2
Reactivity
Bovine
Synonyms
Myosin regulatory light chain 12B; Bovine Myosin regulatory light chain 12B (MYL12B) ELISA Kit; MLC-B; MRLC2; OTTHUMP00000165808|myosin regulatory light chain 2|myosin regulatory light chain MRCL2|myosin regulatory light chain MRLC2; MYL12B elisa kit
Ordering
For Research Use Only!
Reactivity
Bovine
Specificity
This assay has high sensitivity and excellent specificity for detection of Bovine MYL12B. No significant cross-reactivity or interference between Bovine MYL12B and analogues was observed.
Sequence Length
172
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for MYL12B elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MYL12B in samples. An antibody specific for MYL12B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYL12B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYL12B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYL12B bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19,692 Da
NCBI Official Full Name
myosin regulatory light chain 12B
NCBI Official Symbol
MYL12B
NCBI Official Synonym Symbols
MRLC2
NCBI Protein Information
myosin regulatory light chain 12B
UniProt Protein Name
Myosin regulatory light chain 12B
Protein Family
UniProt Gene Name
MYL12B
UniProt Synonym Gene Names
MRLC2; MYLC2B; MLC20

Uniprot Description

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion.

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Product Notes

The Bovine MYL12B myl12b (Catalog #AAA284941) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA284941 ELISA Kit recognizes Bovine MYL12B. It is sometimes possible for the material contained within the vial of "Myosin regulatory light chain 12B, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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