SDS-Page
(MBP protein kinase assay. Myelin Basic Protein was tested in an in vitro kinase assay by incubation with (Lane 1) or without MAPK2 (Lane 2), followed by Western blot detection at 1 ug / lane using an MBP phospho Thr98 antibody. To the right of the Western blot is a lane from a Coomassie-stained SDS-PAGE gel on which 4 ug of purified MBP was loaded.)
Myelin Basic Protein
Myelin Basic Protein, dephosphorylated
Purity
The estimated purity is > 95% based on Coomasie staining.
Synonyms
Myelin Basic; Myelin Basic Protein; dephosphorylated; Myelin Basic protein
Host
Bovine
Purity/Purification
The estimated purity is > 95% based on Coomasie staining.
Form/Format
5mg of MBP (18.5 kDa) at a concentration of 5mg/ml in 10mM MOPS (pH7.0), 0.05% sodium azide, 0.02ug/ml inactive lambda phosphatase, 0.18mM MnCl2, 5mM EDTA.
Protein Species
Bovine
Notes
Myelin Basic Protein, MBP, is useful as a substrate for in vitro kinase assays, including MAPK, PKA, PKC cyclin-dependent and calmodulin-dependent protein kinases.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Lyophilized proteins can be stored at -20 degree C or -80 degree C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(MBP protein kinase assay. Myelin Basic Protein was tested in an in vitro kinase assay by incubation with (Lane 1) or without MAPK2 (Lane 2), followed by Western blot detection at 1 ug / lane using an MBP phospho Thr98 antibody. To the right of the Western blot is a lane from a Coomassie-stained SDS-PAGE gel on which 4 ug of purified MBP was loaded.)
SDS-Page
(MBP protein kinase assay. Myelin Basic Protein was tested in an in vitro kinase assay by incubation with (Lane 1) or without MAPK2 (Lane 2), followed by Western blot detection at 1 ug / lane using an MBP phospho Thr98 antibody. To the right of the Western blot is a lane from a Coomassie-stained SDS-PAGE gel on which 4 ug of purified MBP was loaded.)
Related Product Information for Myelin Basic protein
Short Description: Myelin Basic Protein is purified from bovine brain using SP-Sepharose FPLC and dephosphorylated using lambda phosphatase. Lambda phosphatase is then inactivated by the addition of EDTA and heat treatment (+65C for 1 hour). The estimated purity is >>=95% based on Coomasie staining. Myelin Basic Protein, MBP, is useful as a substrate for in vitro kinase assays, including MAPK.
Product Categories/Family for Myelin Basic protein
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Product Notes
The Myelin Basic (Catalog #AAA388080) is a Protein produced from Bovine and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Myelin Basic, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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