Rabbit anti-Human IRAK1 Polyclonal Antibody | anti-IRAK1 antibody

IRAK1, CT (Interleukin-1 Receptor-associated Kinase 1, IRAK-1, IRAK)

Cat. Num. AAA645654

• Gene Names: IRAK1; IRAK; pelle
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: IRAK1; Polyclonal Antibody; CT (Interleukin-1 Receptor-associated Kinase 1; IRAK-1; IRAK); Anti -IRAK1; anti-IRAK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human IRAK1.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-IRAK1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, ELISA and Immunohistochemistry.; Dilution: ELISA: 1:1000; Western Blot: 1:100-1:500; Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
• Immunogen: Synthetic peptide corresponding to human IRAK1 at C-terminal.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Placenta (formalin-fixed, paraffin-embedded) stained with IRAK1 antibody followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Western Blot (WB)

((LEFT) Western blot analysis of IRAK1 in Jurkat cell lysate. IRAK1 (RIGHT) Western blot analysis of IRAK1 in 293 cell lysates (2ug/lane) either non-transfected (Lane 1) or transiently transfected with the IRAK1 gene (Lane 2).)

Related Product Information for anti-IRAK1 antibody

IRAK1, an IRAK type protein kinase, is a signal transducer for the immune response Toll-like receptor and interleukin-1 (IL-1) receptor signaling cascades. IRAK1 is recruited to the IL-1 receptor, phosphorylated and progressively degraded in response to IL-1 induction. The protein also has the ability to autophosphorylate. IRAK1 is immediately downstream of the MyD88 adapter protein and immediately upstream of TRAF6. IRAK1 (-/-) mice have impaired responses to interleukin-18. At least two mRNA transcript variants have been identified - 2 and 3.6kb.

Product Categories/Family for anti-IRAK1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 68800350
• NCBI GeneID: 3654
• NCBI Accession #: NP_001020414.1
• NCBI GenBank Nucleotide #: NM_001025243.1
• UniProt Accession #: P51617; Q7Z5V4; Q96C06; Q96RL2; D3DWW3; D3DWW4
• Molecular Weight: 76,537 Da
• NCBI Official Full Name: interleukin-1 receptor-associated kinase 1 isoform 3
• NCBI Official Synonym Full Names: interleukin-1 receptor-associated kinase 1
• NCBI Official Symbol: IRAK1
• NCBI Official Synonym Symbols: IRAK; pelle
• NCBI Protein Information: interleukin-1 receptor-associated kinase 1; IRAK-1; Pelle homolog
• UniProt Protein Name: Interleukin-1 receptor-associated kinase 1
• Protein Family: Interleukin-1 receptor-associated kinase
• UniProt Gene Name: IRAK1
• UniProt Synonym Gene Names: IRAK; IRAK-1
• UniProt Entry Name: IRAK1_HUMAN

NCBI Description

This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3. Ref.3 Ref.12 Ref.15 Ref.17 Ref.18 Ref.20 Ref.23 Ref.28

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium.

Subunit structure: Homodimer

By similarity. Interacts with TOLLIP; this interaction occurs in the cytosol prior to receptor activation. Interacts with IL1RL1. Forms a complex with TRAF6, PELI1, IRAK4 and MYD88. Interaction with MYD88 recruits IRAK1 to the stimulated receptor complex. The TRAF6-PELI1-IRAK1-IRAK4-MYD88 complex recruits MAP3K7/TAK1, TAB1 and TAB2 to mediate NF-kappa-B activation. Direct binding of SMAD6 to PELI1 prevents complex formation and hence negatively regulates IL1R-TLR signaling and eventually NF-kappa-B-mediated gene expression. Interacts with IRAK1BP1

By similarity. Interacts (when polyubiquitinated) with IKBKG/NEMO. Interacts with RSAD2/viperin

By similarity. Ref.8 Ref.9 Ref.10 Ref.11 Ref.14 Ref.19 Ref.21 Ref.25

Subcellular location: Cytoplasm. Nucleus. Lipid droplet

By similarity. Note: Translocates to the nucleus when sumoylated. RSAD2/viperin recruits it to the lipid droplet

By similarity. Ref.22

Tissue specificity: Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2. Ref.3

Domain: The ProST region is composed of many proline and serine residues (more than 20 of each) and some threonines. This region is the site of IRAK-1 hyperphosphorylation. Ref.16

Post-translational modification: Following recruitment on the activated receptor complex, phosphorylated on Thr-209, probably by IRAK4, resulting in a conformational change of the kinase domain, allowing further phosphorylations to take place. Thr-387 phosphorylation in the activation loop is required to achieve full enzymatic activity. Ref.3 Ref.13 Ref.15 Ref.16Polyubiquitinated after cell stimulation with IL-1-beta by PELI1, PELI2 and PELI3. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. Ubiquitination promotes interaction with NEMO/IKBKG. Also sumoylated; leading to nuclear translocation. Ref.22

Sequence similarities: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.Contains 1 death domain.Contains 1 protein kinase domain.