V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) Recombinant Protein | MYC recombinant protein

Recombinant V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC)

Cat. Num. AAA2009460

• Gene Names: Myc; Myc2; Nird; Niard; bHLHe39; AU016757
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC); Recombinant V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC); MYC recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-ASECI DPSVVFPYPL NDSSSPKSCT SSDSTAFSPS SDSLLSSESS PRASPEPLVLHEETPPTTSS DSEEEQEDEE EIDVVSVEKR QTPAKRSESG SSPSRGHSKP PHSPLVLKRCHVSTHQHNYA APPSTRKDYP AAKRAKLDSG RVLKQISNNR KCSSPRSSDT EENDKRRTHN VLERQRRNEL KRSFFALRDQ IPELENNEKA PKVVILKKAT AYILSIQADE HKLTSEKDLLRKRREQLKHK LEQLRNSGA
• Sequence Length: 439

• Applicable Applications for MYC recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Ala186~Ala439 (Accession # P01108) with two N-terminal Tags, His-tag and GST-tag
• Subcellular Location: Nucleus, nucleoplasm. nucleolus.
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.8
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 293629264
• NCBI GeneID: 17869
• NCBI Accession #: NP_001170823.1
• NCBI GenBank Nucleotide #: NM_001177352.1
• UniProt Accession #: P01108; P70247; Q3UM70; Q61422
• Molecular Weight: 60.5kDa
• NCBI Official Full Name: myc proto-oncogene protein isoform b
• NCBI Official Synonym Full Names: myelocytomatosis oncogene
• NCBI Official Symbol: Myc
• NCBI Official Synonym Symbols: Myc2; Nird; Niard; bHLHe39; AU016757
• NCBI Protein Information: myc proto-oncogene protein; c-myc proto-oncogene; transcription factor p64
• UniProt Protein Name: Myc proto-oncogene protein
• Protein Family: Viral myc transforming protein
• UniProt Gene Name: Myc
• UniProt Entry Name: MYC_MOUSE

NCBI Description

The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma, in human. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini, in human and mouse. Under conditions of stress, such as high cell densities and methionine deprivation, there is a specific and dramatic increase in the synthesis of the non-AUG initiated protein, suggesting its importance in times of adversity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]

Uniprot Description

Myc: a proto-oncogenic transcription factor that plays a role in cell proliferation, apoptosis and in the development of human tumors. Seems to activate the transcription of growth-related genes.

Protein type: Transcription factor; DNA-binding; Nucleolus; Oncoprotein

Cellular Component: nucleoplasm; nuclear body; protein complex; mitochondrion; axon; cytoplasm; nucleolus; spindle; intracellular; nucleus

Molecular Function: protein dimerization activity; protein binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; double-stranded DNA binding; protein complex binding; transcription factor binding; transcription factor activity

Biological Process: positive regulation of catalytic activity; cellular iron ion homeostasis; positive regulation of transcription, DNA-dependent; transcription initiation; regulation of mitotic cell cycle; positive regulation of caspase activity; B cell apoptosis; Wnt receptor signaling pathway through beta-catenin; middle ear morphogenesis; regulation of apoptosis; positive regulation of fibroblast proliferation; positive regulation of mesenchymal cell proliferation; negative regulation of protein binding; cell cycle arrest; inner mitochondrial membrane organization and biogenesis; positive regulation of B cell apoptosis; transcription, DNA-dependent; regulation of telomere maintenance; positive regulation of cell cycle; glucose metabolic process; pyruvate transport; detection of mechanical stimulus involved in sensory perception of sound; DNA damage response, signal transduction resulting in induction of apoptosis; regulation of gene expression; negative regulation of fibroblast proliferation; regulation of oxidative phosphorylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of epithelial cell proliferation; transcription from RNA polymerase II promoter; response to alkaloid; lactic acid secretion; negative regulation of transcription from RNA polymerase II promoter; chromosome organization and biogenesis; response to radiation; regulation of transcription, DNA-dependent; amino acid transport; positive regulation of cell proliferation; response to gamma radiation; caspase activation; Wnt receptor signaling pathway; MAPKKK cascade; negative regulation of cell division; negative regulation of monocyte differentiation; chromatin remodeling; cell proliferation; negative regulation of glucose import; pigmentation; ureteric bud branching; skeletal morphogenesis; positive regulation of glycolysis; protein processing; response to DNA damage stimulus; transformation of host cell by virus