Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Sialin (SLC17A5) Standard Curve.)
Human Sialin (SLC17A5) ELISA Kit | SIALIN elisa kit
Human Sialin (SLC17A5) ELISA Kit
Gene Names
SLC17A5; SD; AST; NSD; SLD; ISSD; SIASD; SIALIN
Reactivity
Human
Synonyms
Sialin (SLC17A5); Human Sialin (SLC17A5) ELISA Kit; SLC17A5; AST; FLJ22227; FLJ23268; ISSD; NSD; SD; SIASD; SLD; membrane glycoprotein HP59; sialic acid storage disease; sialin; solute carrier family 17; member 5; SIALIN elisa kit
Reactivity
Human
Specificity
Human Sialin (SLC17A5) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC17A5. No significant cross-reactivity or interference between Human SLC17A5 and analogues was observed.
Sequence Length
495
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Sialin (SLC17A5) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Sialin (SLC17A5) Standard Curve.)
Related Product Information for SIALIN elisa kit
Description: This Human Sialin (SLC17A5) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC17A5 in samples. An antibody specific for SLC17A5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC17A5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC17A5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC17A5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The SLC17A5 gene encodes a vesicular excitatory amino acid transporter (VEAT) with dual physiologic functions. When present in synaptic vesicles in the central nervous system, sialin is responsible for vesicular storage and subsequent exocytosis of aspartate and glutamate. When present in lysosomes, it acts as an H(+)-coupled sialic acid exporter. The isolated sequence encoded a predicted 495-residue protein with homology to members of the anion/cation symporter (ACS) family of transporters. This family contains eukaryotic inorganic anion transporters (such as Na+/phosphate cotransporters) as well as prokaryotic organic anion transporters (including H+/acid sugar symporters for hexuronate and glucarate).
Background: The SLC17A5 gene encodes a vesicular excitatory amino acid transporter (VEAT) with dual physiologic functions. When present in synaptic vesicles in the central nervous system, sialin is responsible for vesicular storage and subsequent exocytosis of aspartate and glutamate. When present in lysosomes, it acts as an H(+)-coupled sialic acid exporter. The isolated sequence encoded a predicted 495-residue protein with homology to members of the anion/cation symporter (ACS) family of transporters. This family contains eukaryotic inorganic anion transporters (such as Na+/phosphate cotransporters) as well as prokaryotic organic anion transporters (including H+/acid sugar symporters for hexuronate and glucarate).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
sialin
NCBI Official Synonym Full Names
solute carrier family 17 member 5
NCBI Official Symbol
SLC17A5
NCBI Official Synonym Symbols
SD; AST; NSD; SLD; ISSD; SIASD; SIALIN
NCBI Protein Information
sialin
UniProt Protein Name
Sialin
UniProt Gene Name
SLC17A5
UniProt Synonym Gene Names
AST
UniProt Entry Name
S17A5_HUMAN
NCBI Description
This gene encodes a membrane transporter that exports free sialic acids that have been cleaved off of cell surface lipids and proteins from lysosomes. Mutations in this gene cause sialic acid storage diseases, including infantile sialic acid storage disorder and and Salla disease, an adult form. [provided by RefSeq, Jul 2008]
Research Articles on SIALIN
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Product Notes
The Human SIALIN slc17a5 (Catalog #AAA9714288) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714288 ELISA Kit recognizes Human SIALIN. It is sometimes possible for the material contained within the vial of "Sialin (SLC17A5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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