Rabbit anti-Human, Mouse TAF3 Polyclonal Antibody | anti-TAF3 antibody

TAF3, CT (TAF3, Transcription initiation factor TFIID subunit 3, 140kD TATA box-binding protein-associated factor, TBP-associated factor 3, Transcription initiation factor TFIID 140kD subunit) (PE)

Cat. Num. AAA6353390

• Gene Names: TAF3; TAF140; TAFII140; TAFII-140
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TAF3; Polyclonal Antibody; CT (TAF3; Transcription initiation factor TFIID subunit 3; 140kD TATA box-binding protein-associated factor; TBP-associated factor 3; Transcription initiation factor TFIID 140kD subunit) (PE); Transcription initiation factor TFIID 140kD subunit; anti-TAF3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TAF3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TAF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 613~643 amino acids from the C-terminal region of human TAF3.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TAF3 Antibody (C-term) in NIH-3T3 cell line lysates (35ug/lane). TAF3 (arrow) was detected using the purified Pab.)

Related Product Information for anti-TAF3 antibody

The highly conserved RNA polymerase II transcription factor TFIID (see TAF1; MIM 313650) comprises the TATA box-binding protein (TBP; MIM 600075) and a set of TBP-associated factors (TAFs), including TAF3. TAFs contribute to promoter recognition and selectivity and act as antiapoptotic factors (Gangloff et al., 2001 [PubMed 11438666]).

Product Categories/Family for anti-TAF3 antibody

Antibodies; Transcription Factors

References

Trynka, G., et al. Gut 58(8):1078-1083(2009). Luke, M.M., et al. Stroke 40(2):363-368(2009). Shiffman, D., et al. Arterioscler. Thromb. Vasc. Biol. 28(1):173-179(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 151301171
• NCBI GeneID: 83860
• NCBI Accession #: NP_114129.1
• NCBI GenBank Nucleotide #: NM_031923.3
• UniProt Accession #: Q5VWG9; Q05DA0; Q6GMS5; Q6P6B5; Q86VY6; Q9BQS9; Q9UFI8
• Molecular Weight: 103,582 Da
• NCBI Official Full Name: transcription initiation factor TFIID subunit 3
• NCBI Official Synonym Full Names: TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 140kDa
• NCBI Official Symbol: TAF3
• NCBI Official Synonym Symbols: TAF140; TAFII140; TAFII-140
• NCBI Protein Information: transcription initiation factor TFIID subunit 3; TAF(II)140; TBP-associated factor 3; RNA polymerase II transcription factor TAFII140; 140 kDa TATA box-binding protein-associated factor; transcription initiation factor TFIID 140 kDa subunit
• UniProt Protein Name: Transcription initiation factor TFIID subunit 3
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF3
• UniProt Synonym Gene Names: TAF(II)140; TAF140; TAFII-140; TAFII140
• UniProt Entry Name: TAF3_HUMAN

NCBI Description

The highly conserved RNA polymerase II transcription factor TFIID (see TAF1; MIM 313650) comprises the TATA box-binding protein (TBP; MIM 600075) and a set of TBP-associated factors (TAFs), including TAF3. TAFs contribute to promoter recognition and selectivity and act as antiapoptotic factors (Gangloff et al., 2001 [PubMed 11438666]).[supplied by OMIM, May 2009]

Uniprot Description

TAF140: Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Required in complex with TBPL2 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process. Belongs to the TAF3 family.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 10p15.1

Cellular Component: nucleoplasm; transcription factor TFIID complex; nucleus

Molecular Function: protein binding; zinc ion binding; p53 binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; negative regulation of transcription factor activity; RNA elongation from RNA polymerase II promoter; gene expression; negative regulation of transcription from RNA polymerase II promoter; maintenance of protein localization in nucleus