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Immunohistochemistry - Paraffin (IHC) (Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

Goat RAB27B Polyclonal Antibody | anti-RAB27B antibody

Anti-RAB27B Antibody (C-Terminus) IHC-plus

Gene Names
RAB27B; C25KG
Reactivity
Mouse, Dog, Rat, Human, Monkey
Applications
Immunohistochemistry, Immunohistochemistry, Immunofluorescence, Western Blot
Purity
Immunoaffinity purified
Synonyms
RAB27B; Polyclonal Antibody; Anti-RAB27B Antibody (C-Terminus) IHC-plus; C25KG; Ras-related protein Rab-27B; anti-RAB27B antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Mouse, Dog, Rat, Human, Monkey
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects Rab27b by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab27b. This antibody is specific for Rab27b. It does not recognise Rab27a protein.
Purity/Purification
Immunoaffinity purified
Form/Format
PBS, 20% glycerol, 0.05% sodium azide.
Concentration
3 mg/ml (varies by lot)
Applicable Applications for anti-RAB27B antibody
Immunohistochemistry (IHC), Immunohistochemistry - Paraffin (IHC-P), Immunofluorescence (IF), Western Blot (WB)
Application Notes
IF (1:50 - 1:250)
IHC-P (5 ug/ml)
WB (1:250 - 1:2000)

(optimal dilution to be determined by the researcher)
Immunogen
RAB27B antibody was raised against purified recombinant peptide derived from within residues 120 aa to the C-terminus of mouse Rab27b produced in E Coli.
Anitgen Type
Recombinant protein
Antigen Modification
C-Terminus
Target Species
Mouse
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Immunohistochemistry - Paraffin (IHC)

(Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry - Paraffin (IHC) (Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry - Paraffin (IHC)

(Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry - Paraffin (IHC) (Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot. Anti-Rab27b antibody at 1:500 dilution. Lysates at 100 ug per lane. Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.)

Western Blot (WB) (Western blot. Anti-Rab27b antibody at 1:500 dilution. Lysates at 100 ug per lane. Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ras-related protein Rab-27B
NCBI Official Synonym Full Names
RAB27B, member RAS oncogene family
NCBI Official Symbol
RAB27B
NCBI Official Synonym Symbols
C25KG
NCBI Protein Information
ras-related protein Rab-27B
UniProt Protein Name
Ras-related protein Rab-27B
Protein Family
UniProt Gene Name
RAB27B
UniProt Entry Name
RB27B_HUMAN

NCBI Description

Members of the Rab protein family, including RAB27B, are prenylated, membrane-bound proteins involved in vesicular fusion and trafficking (Chen et al., 1997 [PubMed 9066979]).[supplied by OMIM, Nov 2010]

Uniprot Description

RAB27B: May be involved in targeting uroplakins to urothelial apical membranes. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 18q21.2

Cellular Component: apical plasma membrane; Golgi stack; melanosome; secretory granule; trans-Golgi network transport vesicle; zymogen granule membrane

Molecular Function: GDP binding; GTP binding; GTPase activity; myosin V binding; protein binding; protein domain specific binding

Biological Process: melanosome transport; positive regulation of exocytosis; protein transport; small GTPase mediated signal transduction

Research Articles on RAB27B

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Product Notes

The RAB27B rab27b (Catalog #AAA249686) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Anti-RAB27B Antibody (C-Terminus) IHC-plus reacts with Mouse, Dog, Rat, Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's RAB27B can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunohistochemistry - Paraffin (IHC-P), Immunofluorescence (IF), Western Blot (WB). IF (1:50 - 1:250) IHC-P (5 ug/ml) WB (1:250 - 1:2000) (optimal dilution to be determined by the researcher). Researchers should empirically determine the suitability of the RAB27B rab27b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB27B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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