IFN gamma elispot set

Murine IFN gamma ELISpot Set

Cat. Num. AAA335553

• Gene Names: Ifng; Ifg; IFN-g
• Reactivity: Murine. No cross reactivity with other murine cytokines.
• Synonyms: IFN gamma; Murine IFN gamma ELISpot Set; IFN-g (without plate); IFN gamma elispot set

• Reactivity: Murine. No cross reactivity with other murine cytokines.
• Specificity: Recognizes natural murine IFN-g
• Sequence Length: 155

• Samples: Dilute mouse splenocytes in culture medium and stimulator of interest (i.e. Sample, Vaccine, Peptide pool or infected cells) to give an appropriate cell number per 100ml. Optimal assay performances are observed between 1x105 and 2.5x105 cells per 100ml. Stimulators and incubation times can be varied depending on the frequency of cytokine producing cells and therefore should be optimised by the testing laboratory.

Related Product Information for IFN gamma elispot set

MyBioSource ELISpot Sets include capture and detection antibodies, Streptavidin - Alkaline Phosphatase conjugated, BSA, BCIP/NTB, blocking reagent.

Product Categories/Family for IFN gamma elispot set

Mouse ELISpot Set

References

Adotevi, O.et al., Blood,2010; 115(15): 3025-3032
Bonduelle, O. et al., J. Immunol., 2012; 188: 952 - 956
Chen A. et al., J. Virol., 2005; 79(9): 5568 - 5576

NCBI and Uniprot Product Information

• NCBI GI #: 33468859
• NCBI GeneID: 15978
• NCBI Accession #: NP_032363.1
• NCBI GenBank Nucleotide #: NM_008337.3
• UniProt Accession #: P01580; Q542B8
• Molecular Weight: 17,907 Da
• NCBI Official Full Name: interferon gamma
• NCBI Official Synonym Full Names: interferon gamma
• NCBI Official Symbol: Ifng
• NCBI Official Synonym Symbols: Ifg; IFN-g
• NCBI Protein Information: interferon gamma
• UniProt Protein Name: Interferon gamma
• UniProt Gene Name: Ifng
• UniProt Synonym Gene Names: IFN-gamma
• UniProt Entry Name: IFNG_MOUSE

NCBI Description

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015]

Uniprot Description

IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family.

Protein type: Membrane protein, integral; Secreted; Cytokine; Secreted, signal peptide

Cellular Component: extracellular space; cell; extracellular region; intracellular; external side of plasma membrane

Molecular Function: protein binding; interferon-gamma receptor binding; cytokine activity

Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of cell adhesion; positive regulation of nitric oxide biosynthetic process; apoptosis; positive regulation of osteoclast differentiation; positive regulation of interleukin-12 production; negative regulation of smooth muscle cell proliferation; positive regulation of interleukin-23 production; positive regulation of transcription, DNA-dependent; positive regulation of interleukin-6 biosynthetic process; negative regulation of epithelial cell differentiation; positive regulation of interleukin-1 beta secretion; sensory perception of mechanical stimulus; positive regulation of killing of cells of another organism; negative regulation of transcription from RNA polymerase II promoter; positive regulation of membrane protein ectodomain proteolysis; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; regulation of growth; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; positive regulation of synaptic transmission, cholinergic; neutrophil chemotaxis; regulation of immune response; adaptive immune response; negative regulation of myelination; CD8-positive, alpha-beta T cell differentiation during immune response; unfolded protein response; response to virus; positive regulation of tumor necrosis factor production; defense response to protozoan; humoral immune response; antigen processing and presentation; inflammatory cell apoptosis; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of interleukin-12 biosynthetic process; negative regulation of interleukin-17 production; positive regulation of chemokine biosynthetic process; protein import into nucleus, translocation; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of neuron differentiation; regulation of insulin secretion