MUG recombinant protein
Recombinant E Coli MUG Protein
Gene Names
mug; dug; ECK3058; JW3040; ygjF
Reactivity
E Coli
Applications
ELISA, Western Blot, SDS-Page
Purity
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Synonyms
MUG; Recombinant E Coli MUG Protein; ygjF; G/U mismatch-specific DNA glycosylase; Double-strand-specific uracil glycosylase; Mismatch-specific uracil DNA-glycosylase; MUG recombinant protein
Host
E Coli
Reactivity
E Coli
Purity/Purification
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Form/Format
Supplied as a 0.2 muM filtered solution of 20mM Tris-HCl, 2.5mM beta-ME, 1mM PMSF, 50% Glycerol, pH 8.0.
Sequence
MVEDILAPGL RVVFCGINPG LSSAGTGFPF AHPANRFWKV IYQAGFTDRQ LKPQEAQHLL DYRCGVTKLV DRPTVQANEV SKQELHAGGR KLIEKIEDYQ PQALAILGKQ AYEQGFSQRG AQWGKQTLTI GSTQIWVLPN PSGLSRVSLE KLVEAYRELD QALVVRGRLE HHHHHH
Sequence Length
168
Applicable Applications for MUG recombinant protein
ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
Endotoxin
Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
Directions for Use
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Preparation and Storage
Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.
Related Product Information for MUG recombinant protein
Recombinant E Coli MUG Protein is produced by our E.coli expression system and the target gene encoding Met1-Arg168 is expressed with a 6His tag at the C-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,673 Da
NCBI Official Full Name
G/U mismatch-specific DNA glycosylase; xanthine DNA glycosylase
NCBI Official Symbol
mug
NCBI Official Synonym Symbols
dug; ECK3058; JW3040; ygjF
NCBI Protein Information
G/U mismatch-specific DNA glycosylase; xanthine DNA glycosylase
UniProt Protein Name
G/U mismatch-specific DNA glycosylase
Protein Family
UniProt Gene Name
mug
UniProt Synonym Gene Names
ygjF; MUG
UniProt Entry Name
MUG_ECOLI
NCBI Description
Endonuclease IV stimulates Mug activity in vitro by promoting disassociation from the DNA and enzyme turnover. [More information is available at EcoGene: EG12717]. Mug is a glycosylase that acts in DNA repair . [More information is available at EcoCyc: EG12717].
Uniprot Description
Excises ethenocytosine and uracil, which can arise by alkylation or deamination of cytosine, respectively, from the corresponding mispairs with guanine in ds-DNA. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Required for DNA damage lesion repair in stationary-phase cells.
Research Articles on MUG
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Product Notes
The MUG mug (Catalog #AAA8249471) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant E Coli MUG Protein reacts with E Coli and may cross-react with other species as described in the data sheet. AAA Biotech's MUG can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS). Researchers should empirically determine the suitability of the MUG mug for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MVEDILAPGL RVVFCGINPG LSSAGTGFPF AHPANRFWKV IYQAGFTDRQ LKPQEAQHLL DYRCGVTKLV DRPTVQANEV SKQELHAGGR KLIEKIEDYQ PQALAILGKQ AYEQGFSQRG AQWGKQTLTI GSTQIWVLPN PSGLSRVSLE KLVEAYRELD QALVVRGRLE HHHHHH. It is sometimes possible for the material contained within the vial of "MUG, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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