Human Mdm2-binding protein ELISA Kit | MTBP elisa kit

Human Mdm2-binding protein ELISA Kit

Cat. Num. AAA762916

• Gene Names: MTBP; MDM2BP
• Reactivity: Human
• Synonyms: Mdm2-binding protein; Human Mdm2-binding protein ELISA Kit; MTBP/Mdm2-binding protein/hMTBP/MDM2BP; MTBP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MTBP. No significant cross-reactivity or interference between MTBP and analogues was observed.
• Sequence Length: 904

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 0.312-20ng/ml
• Sensitivity: <0.188ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MTBP were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MTBP were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MTBP elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MTBP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- MTBP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MTBP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MTBP can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 21630257
• NCBI GeneID: 27085
• NCBI Accession #: NP_071328.2
• NCBI GenBank Nucleotide #: NM_022045.4
• Molecular Weight: 37,253 Da
• NCBI Official Full Name: mdm2-binding protein
• NCBI Official Synonym Full Names: MDM2 binding protein
• NCBI Official Symbol: MTBP
• NCBI Official Synonym Symbols: MDM2BP
• NCBI Protein Information: mdm2-binding protein
• UniProt Protein Name: Mdm2-binding protein
• Protein Family: Mdm2-binding protein
• UniProt Gene Name: MTBP
• UniProt Synonym Gene Names: hMTBP
• UniProt Entry Name: MTBP_HUMAN

NCBI Description

This gene encodes a protein that interacts with the oncoprotein mouse double minute 2. The encoded protein regulates progression through the cell cycle and may be involved in tumor formation. [provided by RefSeq, Aug 2012]

Uniprot Description

MTBP: Inhibits cell migration in vitro and suppresses the invasive behavior of tumor cells. May play a role in MDM2-dependent p53/TP53 homeostasis in unstressed cells. Inhibits autoubiquitination of MDM2, thereby enhancing MDM2 stability. This promotes MDM2-mediated ubiquitination of p53/TP53 and its subsequent degradation. Belongs to the MTBP family.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 8q24.12

Cellular Component: chromatin; kinetochore

Biological Process: cell cycle arrest; negative regulation of cell proliferation; negative regulation of mitosis; traversing start control point of mitotic cell cycle