Typical Testing Data/Standard Curve (for reference only)
Human mesothelin ELISA Kit | MSLN elisa kit
Human Mesothelin, MSLN ELISA Kit
Gene Names
MSLN; MPF; SMRP
Reactivity
Human
Synonyms
mesothelin; Human Mesothelin; MSLN ELISA Kit; Human Mesothelin (MSLN) ELISA KIT; CAK1; MPF; SMR; megakaryocyte potentiating factor; MSLN elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human MSLN. No significant cross-reactivity or interference between Human MSLN and analogues was observed.
Sequence Length
622
Detection Range
0.156 ng/ml-10 ng/ml
Sensitivity
0.04 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Cell Adhesion
Protein Biological Process 3
Cell adhesion
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MSLN elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MSLN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for MSLN and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain MSLN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MSLN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,986 Da
NCBI Official Full Name
mesothelin isoform 1 preproprotein
NCBI Official Synonym Full Names
mesothelin
NCBI Official Symbol
MSLN
NCBI Official Synonym Symbols
MPF; SMRP
NCBI Protein Information
mesothelin; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor
UniProt Protein Name
Mesothelin
Protein Family
UniProt Gene Name
MSLN
UniProt Synonym Gene Names
MPF; MPF
UniProt Entry Name
MSLN_HUMAN
NCBI Description
This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]
Uniprot Description
Function: Membrane-anchored forms may play a role in cellular adhesion.2 PublicationsManual assertion based on experiment in:Ref.9
Research Articles on MSLN
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Product Notes
The Human MSLN msln (Catalog #AAA702446) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA702446 ELISA Kit recognizes Human MSLN. It is sometimes possible for the material contained within the vial of "mesothelin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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