Human DNASE2 (Deoxyribonuclease-2-alpha) ELISA Kit | DNASE2 elisa kit

Human DNASE2 (Deoxyribonuclease-2-alpha) ELISA Kit

Cat. Num. AAA7608177

• Gene Names: DNASE2; DNL; DNL2; DNASE2A
• Reactivity: Human
• Synonyms: DNASE2 (Deoxyribonuclease-2-alpha); Human DNASE2 (Deoxyribonuclease-2-alpha) ELISA Kit; Acid DNase; Deoxyribonuclease 2 alpha; Deoxyribonuclease II alpha; DNase II alpha; DNASE2; DNASE2A; DNL; DNL2; Lysosomal DNase II; DNASE2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of DNASE2. No significant cross-reactivity or interference between DNASE2 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DNASE2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 2921838
• NCBI GeneID: 1777
• UniProt Accession #: O00115; O43910; B2RD06; B7Z4K6
• Molecular Weight: Aprox: 40 kDa
• NCBI Official Full Name: Homo sapiens deoxyribonuclease II alpha mRNA, complete cds
• NCBI Official Synonym Full Names: deoxyribonuclease II, lysosomal
• NCBI Official Symbol: DNASE2
• NCBI Official Synonym Symbols: DNL; DNL2; DNASE2A
• NCBI Protein Information: deoxyribonuclease-2-alpha; R31240_2; acid DNase; DNase II alpha; lysosomal DNase II; DNase II, lysosomal; deoxyribonuclease II alpha
• UniProt Protein Name: Deoxyribonuclease-2-alpha
• Protein Family: Deoxyribonuclease
• UniProt Gene Name: DNASE2
• UniProt Synonym Gene Names: DNASE2A; DNL2; DNase II alpha
• UniProt Entry Name: DNS2A_HUMAN

NCBI Description

This gene encodes a member of the DNase family. The protein, located in the lysosome, hydrolyzes DNA under acidic conditions and mediates the breakdown of DNA during erythropoiesis and apoptosis. Two codominant alleles have been characterized, DNASE2*L (low activity) and DNASE2*H (high activity), that differ at one nucleotide in the promoter region. The DNASE2*H allele is represented in this record. [provided by RefSeq, Jul 2008]

Uniprot Description

DNASE2: Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells. Belongs to the DNase II family.

Protein type: Deoxyribonuclease; EC 3.1.22.1

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: lysosome; intracellular

Molecular Function: DNA binding; deoxyribonuclease II activity

Biological Process: erythrocyte differentiation; DNA fragmentation during apoptosis; DNA metabolic process