Mouse Mesothelin ELISA Kit | Msln elisa kit

Mouse Mesothelin ELISA Kit

Cat. Num. AAA760334

• Gene Names: MSLN; MPF; SMRP
• Reactivity: Mouse
• Synonyms: Mesothelin; Mouse Mesothelin ELISA Kit; Msln/Mesothelin/MSLN/CAK1/MPF/MSLN/SMR/CAK1/CAK1 antigen/megakaryocyte potentiating factor/MPFSMRP/Pre-pro-megakaryocyte-potentiating factor/soluble MPF mesothelin related protein; Msln elisa kit

• Reactivity: Mouse
• Sequence Length: 622

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Msln elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Msln antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Msln antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Msln amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Msln can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 293651530
• NCBI GeneID: 10232
• NCBI Accession #: NP_001170826.1
• NCBI GenBank Nucleotide #: NM_001177355.2
• Molecular Weight: 67,940 Da
• NCBI Official Full Name: mesothelin isoform 1 preproprotein
• NCBI Official Synonym Full Names: mesothelin
• NCBI Official Symbol: MSLN
• NCBI Official Synonym Symbols: MPF; SMRP
• NCBI Protein Information: mesothelin
• UniProt Protein Name: Mesothelin
• Protein Family: Mesothelin
• UniProt Gene Name: MSLN
• UniProt Synonym Gene Names: MPF; MPF
• UniProt Entry Name: MSLN_HUMAN

NCBI Description

This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]

Uniprot Description

MSLN: Membrane-anchored forms may play a role in cellular adhesion. Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. Belongs to the mesothelin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Cell adhesion

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cell surface; extracellular space; Golgi apparatus; membrane; plasma membrane

Molecular Function: protein binding

Biological Process: cell adhesion; cell-matrix adhesion; pancreas development