anti-Human Caspase 10 Antibody | anti-CASP-10 antibody

Caspase 10 Antibody

Cat. Num. AAA851625

• Gene Names: CASP10; MCH4; ALPS2; FLICE2
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: Caspase 10; Caspase 10 Antibody; ALPS2; CASP10B; FLICE2; Caspase 10 isoform a preproprotein; Caspase 10 isoform b preproprotein; Caspase 10 isoform c preproprotein; Caspase 10 isoform d preproprotein; FADD-LIKE ICE 2; FADD-like ICE2; Fas-associated death domain protein; ICE-like apoptotic protease 4; MCH4 CASPASE 10; ISOFORM B; apoptotic protease MCH-4; caspase 10; apoptosis-related cysteine protease; interleukin-1B-converting enzyme 2; anti-CASP-10 antibody

• Reactivity: Human
• Specificity: Caspase 10 antibody detects endogenous levels of total Caspase 10 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 455

• Applicable Applications for anti-CASP-10 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:300~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase 10.
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Caspase 10 antibody.)

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, using Caspase 10 antibody.)

Immunofluorescence (IF)

(Peptide-+ Immunofluorescence analysis of HeLa cells, using Caspase 10 antibody.)

References

Frank C. Kischkel, J. Biol. Chem., Nov 2001; 276: 46639-46646. Ingo H. Engels, Mol. Cell. Biol., Apr 2005; 25: 2808-2818. Daniela Sciaky, J. Immunol., Apr 2000; 164: 3806. Jin Wang, PNAS, Nov 2001; 98: 13884.

NCBI and Uniprot Product Information

• NCBI GI #: 330864823
• NCBI GeneID: 843
• NCBI Accession #: NP_001193453.1
• NCBI GenBank Nucleotide #: NM_001206524.1
• UniProt Accession #: Q92851; Q68HC0; Q6KF62; Q6KF63; Q8IUP5; Q8WYQ8; Q99845; Q9Y2U6; Q9Y2U7
• Molecular Weight: 28,364 Da
• NCBI Official Full Name: caspase-10 isoform 6 preproprotein
• NCBI Official Synonym Full Names: caspase 10
• NCBI Official Symbol: CASP10
• NCBI Official Synonym Symbols: MCH4; ALPS2; FLICE2
• NCBI Protein Information: caspase-10
• UniProt Protein Name: Caspase-10
• UniProt Gene Name: CASP10
• UniProt Synonym Gene Names: MCH4; CASP-10; FLICE2

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011]

Uniprot Description

Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC.