Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using FGFR3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit FGF Receptor 3 Polyclonal Antibody | anti-FGFR3 antibody
FGF Receptor 3 Rabbit pab Antibody
Gene Names
FGFR3; ACH; CEK2; JTK4; CD333; HSFGFR3EX
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Synonyms
FGF Receptor 3; Polyclonal Antibody; FGF Receptor 3 Rabbit pab Antibody; FGFR3; FGF Receptor 3 ; fibroblast growth factor receptor 3; JTK4; CD333; CEK2; ACH;; anti-FGFR3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Liquid
Sequence Length
806
Applicable Applications for anti-FGFR3 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot (WB): 1/200-500
Immunohistochemistry (IHC): 1/50-100
Immunohistochemistry (IHC): 1/50-100
Immunogen
A synthetic -peptide of human FGF Receptor 3
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol,
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using FGFR3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using FGFR3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Related Product Information for anti-FGFR3 antibody
Rabbit polyclonal to Human FGF Receptor 3
Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3, and FGFR-4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR-1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCgamma (4,5). FGFR-3 participates in a diverse array of biological processes, including cell growth, differentiation, and migration. Activating mutations in FGFR-3 are associated with multiple myeloma, cervical carcinoma, and bladder cancer and it represents a potential target for therapy (6).
Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3, and FGFR-4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR-1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCgamma (4,5). FGFR-3 participates in a diverse array of biological processes, including cell growth, differentiation, and migration. Activating mutations in FGFR-3 are associated with multiple myeloma, cervical carcinoma, and bladder cancer and it represents a potential target for therapy (6).
References
1. Powers, C.J. et al. (2000) Endocr Relat Cancer 7, 165-97. 2. Reilly, J.F. et al. (2000) J Biol Chem 275, 7771-8. 3. Mohammadi, M. et al. (1996) Mol Cell Biol 16, 977-89. 4. Mohammadi, M. et al. (1991) Mol Cell Biol 11, 5068-78. 5. Larsson, H. et al. (1999) J Biol Chem 274, 25726-34. 6. Knowles, M.A. (2008) Future Oncol 4, 71-83.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
806
NCBI Official Full Name
fibroblast growth factor receptor 3 isoform 1
NCBI Official Synonym Full Names
fibroblast growth factor receptor 3
NCBI Official Symbol
FGFR3
NCBI Official Synonym Symbols
ACH; CEK2; JTK4; CD333; HSFGFR3EX
NCBI Protein Information
fibroblast growth factor receptor 3; FGFR-3; tyrosine kinase JTK4; hydroxyaryl-protein kinase; fibroblast growth factor receptor 3 variant 4
UniProt Protein Name
Fibroblast growth factor receptor 3
Protein Family
UniProt Gene Name
FGFR3
UniProt Synonym Gene Names
JTK4; FGFR-3
UniProt Entry Name
FGFR3_HUMAN
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Product Notes
The FGFR3 fgfr3 (Catalog #AAA125314) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FGF Receptor 3 Rabbit pab Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FGF Receptor 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot (WB): 1/200-500 Immunohistochemistry (IHC): 1/50-100. Researchers should empirically determine the suitability of the FGFR3 fgfr3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FGF Receptor 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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