Typical Testing Data/Standard Curve (for reference only)
Mouse Spermidine/Spermine N1-Acetyltransferase 1 (SAT1) RTU ELISA Kit | SAT1 rtu elisa kit
Mouse Spermidine/Spermine N1-Acetyltransferase 1 (SAT1) ELISA Kit
Gene Names
Sat1; Sat; SSAT; AA617398
Reactivity
Mouse
Synonyms
Spermidine/Spermine N1-Acetyltransferase 1 (SAT1); Mouse Spermidine/Spermine N1-Acetyltransferase 1 (SAT1) ELISA Kit; DC21; KFSD; SAT; SSAT; SSAT-1; Diamine Acetyltransferase 1; Polyamine N-acetyltransferase 1; Putrescine acetyltransferase; SAT1 rtu elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of S100A4. No significant cross-reactivity or interference between S100A4 and analogues was observed.
Sequence Length
96
Samples
Human Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates Or Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
78.1-5000pg/mL
Sensitivity
29pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100A4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100A4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SAT1 rtu elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of S100A4 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100A4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to S100A4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100A4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of S100A4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100A4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to S100A4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100A4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of S100A4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,012 Da
NCBI Official Full Name
diamine acetyltransferase 1 isoform 2
NCBI Official Synonym Full Names
spermidine/spermine N1-acetyl transferase 1
NCBI Official Symbol
Sat1
NCBI Official Synonym Symbols
Sat; SSAT; AA617398
NCBI Protein Information
diamine acetyltransferase 1
UniProt Protein Name
Diamine acetyltransferase 1
Protein Family
UniProt Gene Name
Sat1
UniProt Synonym Gene Names
Sat; SSAT; SSAT-1
Uniprot Description
Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine = spermidine >> spermine > N1-acetylspermine > putrescine. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Also involved in the regulation of polyamine transport out of cells. Acts on 1,3-diaminopropane, 1,5-diaminopentane, putrescine, spermidine (forming N1- and N8-acetylspermidine), spermine, N1-acetylspermidine and N8-acetylspermidine ().
Research Articles on SAT1
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Product Notes
The Mouse SAT1 sat1 (Catalog #AAA4502356) is a RTU ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA4502356 RTU ELISA Kit recognizes Mouse SAT1. It is sometimes possible for the material contained within the vial of "Spermidine/Spermine N1-Acetyltransferase 1 (SAT1), RTU ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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