Rat Spermidine/Spermine N1-Acetyltransferase 1 (SAT1) ELISA Kit | SAT1 elisa kit

Rat Spermidine/Spermine N1-Acetyltransferase 1 (SAT1) ELISA Kit

Cat. Num. AAA2706232

• Gene Names: SAT1; SAT; DC21; KFSD; SSAT; KFSDX; SSAT-1
• Reactivity: Rat
• Synonyms: Spermidine/Spermine N1-Acetyltransferase 1 (SAT1); Rat Spermidine/Spermine N1-Acetyltransferase 1 (SAT1) ELISA Kit; DC21; KFSD; SAT; SSAT; SSAT-1; Diamine Acetyltransferase 1; Polyamine N-acetyltransferase 1; Putrescine acetyltransferase; SAT1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Spermidine/Spermine N1-Acetyltransferase 1 (SAT1).; No significant cross-reactivity or interference between Spermidine/Spermine N1-Acetyltransferase 1 (SAT1) and analogues was observed.
• Sequence Length: 171

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.113ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Spermidine/Spermine N1-Acetyltransferase 1 (SAT1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Spermidine/Spermine N1-Acetyltransferase 1 (SAT1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SAT1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Spermidine/Spermine N1-Acetyltransferase 1 (SAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Spermidine/Spermine N1-Acetyltransferase 1 (SAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Spermidine/Spermine N1-Acetyltransferase 1 (SAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Spermidine/Spermine N1-Acetyltransferase 1 (SAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 14250050
• NCBI GeneID: 6303
• UniProt Accession #: P21673; Q6ICU9
• Molecular Weight: 20,024 Da
• NCBI Official Full Name: Spermidine/spermine N1-acetyltransferase 1
• NCBI Official Synonym Full Names: spermidine/spermine N1-acetyltransferase 1
• NCBI Official Symbol: SAT1
• NCBI Official Synonym Symbols: SAT; DC21; KFSD; SSAT; KFSDX; SSAT-1
• NCBI Protein Information: diamine acetyltransferase 1
• UniProt Protein Name: Diamine acetyltransferase 1
• Protein Family: Serine acetyltransferase
• UniProt Gene Name: SAT1
• UniProt Synonym Gene Names: SAT; SSAT; SSAT-1

NCBI Description

The protein encoded by this gene belongs to the acetyltransferase family, and is a rate-limiting enzyme in the catabolic pathway of polyamine metabolism. It catalyzes the acetylation of spermidine and spermine, and is involved in the regulation of the intracellular concentration of polyamines and their transport out of cells. Defects in this gene are associated with keratosis follicularis spinulosa decalvans (KFSD). Alternatively spliced transcripts have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine = spermidine >> spermine > N1-acetylspermine > putrescine. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Also involved in the regulation of polyamine transport out of cells. Acts on 1,3-diaminopropane, 1,5-diaminopentane, putrescine, spermidine (forming N1- and N8-acetylspermidine), spermine, N1-acetylspermidine and N8-acetylspermidine.