Mouse Complement Component 5 (C5) RTU ELISA Kit | C5 rtu elisa kit

Mouse Complement Component 5 (C5) ELISA Kit

Cat. Num. AAA458041

• Gene Names: C5; C5D; C5a; C5b; ECLZB; CPAMD4
• Reactivity: Mouse
• Synonyms: Complement Component 5 (C5); Mouse Complement Component 5 (C5) ELISA Kit; CPAMD4; C5a anaphylatoxin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; C5 rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 1676

• Assay Type: Sandwich
• Samples: Serum, Plasma or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.059ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C5 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to C5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to C5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 38016947
• NCBI GeneID: 727
• NCBI Accession #: NP_001726.2
• NCBI GenBank Nucleotide #: NM_001735.2
• UniProt Accession #: P01031; Q14CJ0; Q27I61
• Molecular Weight: 188,305 Da
• NCBI Official Full Name: complement C5 isoform 1 preproprotein
• NCBI Official Synonym Full Names: complement C5
• NCBI Official Symbol: C5
• NCBI Official Synonym Symbols: C5D; C5a; C5b; ECLZB; CPAMD4
• NCBI Protein Information: complement C5
• UniProt Protein Name: Complement C5
• Protein Family: Complement C5
• UniProt Gene Name: C5
• UniProt Synonym Gene Names: CPAMD4

NCBI Description

This gene encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the C5 alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mutations in this gene cause complement component 5 deficiency, a disease characterized by recurrent bacterial infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2015]

Uniprot Description

Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed:8182049). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.