Rabbit NCF2 Polyclonal Antibody | anti-NCF2 antibody

NCF2 (Neutrophil Cytosol Factor 2, FLJ93058, NCF-2, NOXA2, P67-PHOX, P67PHOX) (FITC)

Cat. Num. AAA6428325

• Gene Names: NCF2; NCF-2; NOXA2; P67PHOX; P67-PHOX
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: NCF2; Polyclonal Antibody; NCF2 (Neutrophil Cytosol Factor 2; FLJ93058; NCF-2; NOXA2; P67-PHOX; P67PHOX) (FITC); anti-NCF2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NCF2. Species Crossreactivity: mouse and rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-NCF2 antibody: Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Recombinant fusion protein corresponding to aa227-526 of human NOXA2 / P67phox.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using 212198 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human esophageal cancer using NCF2 antibody at dilution of 1:100 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human esophagus using NCF2 antibody at dilution of 1:100 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using NCF2 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-NCF2 antibody

p67phox appears to coordinate assembly of NAPDH oxidase as it associates with multiple subunits as well as the alpha subunit of heterotrimeric G proteins (7). Mutations in the corresponding p67phox gene are also associated with a form of autosomal recessive chronic granulomatous disease (8).

Product Categories/Family for anti-NCF2 antibody

Antibodies; Enzymes; Oxidase

NCBI and Uniprot Product Information

• NCBI GI #: 1346669
• NCBI GeneID: 4688
• UniProt Accession #: P19878; Q2PP06; Q8NFC7; Q9BV51; B2R6Q1; B4DKQ7; B4DQA7; E9PHJ2; E9PHX3
• Molecular Weight: 59,762 Da
• NCBI Official Full Name: Neutrophil cytosol factor 2
• NCBI Official Synonym Full Names: neutrophil cytosolic factor 2
• NCBI Official Symbol: NCF2
• NCBI Official Synonym Symbols: NCF-2; NOXA2; P67PHOX; P67-PHOX
• NCBI Protein Information: neutrophil cytosol factor 2; NADPH oxidase activator 2; 67 kDa neutrophil oxidase factor; neutrophil NADPH oxidase factor 2; chronic granulomatous disease, autosomal 2; neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2)
• UniProt Protein Name: Neutrophil cytosol factor 2
• Protein Family: Neutrophil cytosol factor
• UniProt Gene Name: NCF2
• UniProt Synonym Gene Names: NOXA2; P67PHOX; NCF-2
• UniProt Entry Name: NCF2_HUMAN

NCBI Description

This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]

Uniprot Description

p67phox: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). Interacts with SYTL1 and RAC1. Interacts with NCF4. May interact with NOXO1. Belongs to the NCF2/NOXA1 family.

Protein type: Oxidoreductase

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: cytoplasm; nucleolus; acrosome; cytosol; NADPH oxidase complex

Molecular Function: protein C-terminus binding; protein binding; electron carrier activity; Rac GTPase binding

Biological Process: respiratory burst; response to drug; interaction with host; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; response to lipopolysaccharide; cellular response to hormone stimulus; antigen processing and presentation of peptide antigen via MHC class I; response to hyperoxia; positive regulation of neuron apoptosis; response to glucose stimulus; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; cellular defense response; response to activity; response to progesterone stimulus; vascular endothelial growth factor receptor signaling pathway; superoxide release; positive regulation of blood pressure; aging

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type Ii