Flow Cytometry (FC/FACS)
(Rat splenocytes are stained with Anti-Rat CD3 Monoclonal Antibody(APC Conjugated)[Used at 0.05 mug/106 cells dilution](filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)
Mouse anti-Rat CD3 Antibody | anti-CD3 antibody
Anti-Rat CD3 Monoclonal Antibody (APC Conjugated)
Reactivity
Rat
Applications
Flow Cytometry, Functional Assay
Synonyms
CD3; Anti-Rat CD3 Monoclonal Antibody (APC Conjugated); Rat CD3; T-cell surface glycoprotein CD3 delta; gamma; epsilon; and zeta chains; CD3 Complex; T3; anti-CD3 antibody
Host
Mouse
Reactivity
Rat
Isotype
IgG3, kappa
Clone Number
G4.18
Form/Format
PBS with 0.05% Proclin300, 1% BSA
Concentration
0.2 mg/mL (varies by lot)
Sequence Length
173
Applicable Applications for anti-CD3 antibody
Flow Cytometry (FC/FACS)
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results or refer to the dilution we used in the experiment.
Conjugation
APC
Preparation and Storage
Store at 2-8 degree C and protected from prolonged exposure to light. Do not freeze.
Flow Cytometry (FC/FACS)
(Rat splenocytes are stained with Anti-Rat CD3 Monoclonal Antibody(APC Conjugated)[Used at 0.05 mug/106 cells dilution](filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)
Flow Cytometry (FC/FACS)
(Rat splenocytes are stained with Anti-Rat CD3 Monoclonal Antibody(APC Conjugated)[Used at 0.05 mug/106 cells dilution](filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)
Related Product Information for anti-CD3 antibody
CD3 is a complex composed of delta, gamma, epsilon, and zeta chains. They are 20-25 kD members of the immunoglobulin superfamily and associated with the T cell receptor (TCR). CD3 is expressed on thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells. CD3 is involved in antigen recognition, signal transduction, and T cell activation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
T-cell surface glycoprotein CD3 delta chain
NCBI Official Synonym Full Names
CD3d molecule
NCBI Official Symbol
Cd3d
NCBI Protein Information
T-cell surface glycoprotein CD3 delta chain
UniProt Protein Name
T-cell surface glycoprotein CD3 delta chain
Protein Family
UniProt Gene Name
Cd3d
UniProt Synonym Gene Names
T3d
UniProt Entry Name
CD3D_RAT
NCBI Description
putative component of the CD3 T-cell receptor [RGD, Feb 2006]
Similar Products
Product Notes
The CD3 cd3d (Catalog #AAA2559617) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rat CD3 Monoclonal Antibody (APC Conjugated) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results or refer to the dilution we used in the experiment. Researchers should empirically determine the suitability of the CD3 cd3d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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