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Flow Cytometry (FC/FACS) (C57BL/6 murine splenocytes are stained with Anti-Mouse CD3epsilon Monoclonal Antibody(FITC Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)

anti-Mouse CD3epsilon Monoclonal Antibody | anti-CD3E antibody

Anti-Mouse CD3epsilon Monoclonal Antibody (FITC Conjugated) [145-2C11]

Gene Names
Cd3e; CD3; T3e; AI504783; CD3epsilon
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Synonyms
CD3epsilon; Monoclonal Antibody; Anti-Mouse CD3epsilon Monoclonal Antibody (FITC Conjugated) [145-2C11]; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e; CD3E; T3E; anti-CD3E antibody
Ordering
For Research Use Only!
Host
Armenian Hamster
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG
Clone Number
145-2C11
Form/Format
PBS with 0.05% Proclin300, 1% BSA
Sequence Length
189
Applicable Applications for anti-CD3E antibody
Flow Cytometry (FC/FACS)
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 2 muL or 5 muL of antibody per test (million cells in 100 ul staining volume or per 100 ul of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Conjugation
FITC
Preparation and Storage
Store at 2-8 degree C and protect from prolonged exposure to light. Do not freeze.

Flow Cytometry (FC/FACS)

(C57BL/6 murine splenocytes are stained with Anti-Mouse CD3epsilon Monoclonal Antibody(FITC Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)

Flow Cytometry (FC/FACS) (C57BL/6 murine splenocytes are stained with Anti-Mouse CD3epsilon Monoclonal Antibody(FITC Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)
Related Product Information for anti-CD3E antibody
CD3epsilon is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3epsilon forms a TCR complex by associating with the CD3delta, gamma and zeta chains, as well as the TCR alpha/beta or gamma/delta chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
T-cell surface glycoprotein CD3 epsilon chain
NCBI Official Synonym Full Names
CD3 antigen, epsilon polypeptide
NCBI Official Symbol
Cd3e
NCBI Official Synonym Symbols
CD3; T3e; AI504783; CD3epsilon
NCBI Protein Information
T-cell surface glycoprotein CD3 epsilon chain
UniProt Protein Name
T-cell surface glycoprotein CD3 epsilon chain
UniProt Gene Name
Cd3e
UniProt Entry Name
CD3E_MOUSE

Research Articles on CD3E

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Product Notes

The CD3E cd3e (Catalog #AAA2558690) is an Antibody produced from Armenian Hamster and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse CD3epsilon Monoclonal Antibody (FITC Conjugated) [145-2C11] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD3epsilon can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 2 muL or 5 muL of antibody per test (million cells in 100 ul staining volume or per 100 ul of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. Researchers should empirically determine the suitability of the CD3E cd3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3epsilon, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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