Rat anti-Mouse CD3 Monoclonal Antibody | anti-CD3 antibody

Anti-Mouse CD3 Monoclonal Antibody (FITC Conjugated)

Cat. Num. AAA2558083

• Gene Names: Cd3d; T3d
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD3; Monoclonal Antibody; Anti-Mouse CD3 Monoclonal Antibody (FITC Conjugated); T-cell surface glycoprotein CD 3epsilon/delta/gamma/zeta chain; CD3E/D/G/Z; CD3e/d/g/z; anti-CD3 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: 17A2
• Form/Format: PBS with 0.05% Proclin300, 1% BSA
• Concentration: 0.5 mg/mL (varies by lot)
• Sequence Length: 173

• Applicable Applications for anti-CD3 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is <= 2 ug per 10⁶ cells in 100 ul volume or 100 ul of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
• Conjugation: FITC
• Preparation and Storage: Store at 2-8 degree C and protect from prolonged exposure to light. Do not freeze.

Flow Cytometry (FC/FACS)

(Mouse splenocytes are stained with Anti-Mouse CD3 Monoclonal Antibody(FITC Conjugated)(filled gray curve). Unstained splenocytes (blank black curve) are used as control.)

Related Product Information for anti-CD3 antibody

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3epsilon, delta, gamma and zeta chains. It forms a TCR complex by associating with TCR alpha/beta or gamma/delta chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

NCBI and Uniprot Product Information

• NCBI GI #: 227498961
• NCBI GeneID: 12500
• NCBI Accession #: NP_038515.3
• NCBI GenBank Nucleotide #: NP_038515.3
• UniProt Accession #: P04235; P11942; P22646; P24161; P29020
• NCBI Official Full Name: T-cell surface glycoprotein CD3 delta chain
• NCBI Official Synonym Full Names: CD3 antigen, delta polypeptide
• NCBI Official Symbol: Cd3d
• NCBI Official Synonym Symbols: T3d
• NCBI Protein Information: T-cell surface glycoprotein CD3 delta chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 delta chain
• Protein Family: CD302 antigen
• UniProt Gene Name: Cd3d
• UniProt Synonym Gene Names: T3d
• UniProt Entry Name: CD3D_MOUSE

Uniprot Description

CD3D: a T cell surface glycoprotein that is a component of the T cell antigen receptor. A defect in this protein causes a severe combined immunodeficiency characterized by the absence of T cells but normal B cells. Contains 1 ITAM domain.

Protein type: Receptor, misc.; Membrane protein, integral

Cellular Component: T cell receptor complex; membrane; integral to membrane; alpha-beta T cell receptor complex

Molecular Function: protein binding; transmembrane receptor activity; protein heterodimerization activity

Biological Process: cell surface receptor linked signal transduction; T cell activation; protein complex assembly; positive thymic T cell selection