Western Blot (WB)
(Figure 1. Western blot analysis of SHISA7 using anti-SHISA7 antibody (MBS1753846).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysatesLane 2: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-SHISA7 antigen affinity purified polyclonal antibody (Catalog # MBS1753846) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for SHISA7 at approximately 70KD. The expected band size for SHISA7 is at 70KD. )
Rabbit SHISA7 Polyclonal Antibody | anti-SHISA7 antibody
Anti-SHISA7 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Immunogen affinity purified.
Synonyms
SHISA7; Polyclonal Antibody; Anti-SHISA7 Antibody; Protein shisa-7; Cystine-knot AMPAR modulating protein of 59 kDa; CKAMP59; GABA(A) receptor auxiliary subunit Shisa7; Protein shisa-6-like; shisa family member 7; anti-SHISA7 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rabbit IgG polyclonal antibody for SHISA7 detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Applicable Applications for anti-SHISA7 antibody
Western Blot (WB), Direct ELISA (EIA)
Application Notes
WB: 0.25-0.5ug/ml|Mouse, Rat|
Direct ELISA: 0.1-0.5ug/ml|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Immunogen
E Coli-derived human SHISA7 recombinant protein (Position: Y308-E535).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Figure 1. Western blot analysis of SHISA7 using anti-SHISA7 antibody (MBS1753846).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysatesLane 2: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-SHISA7 antigen affinity purified polyclonal antibody (Catalog # MBS1753846) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for SHISA7 at approximately 70KD. The expected band size for SHISA7 is at 70KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of SHISA7 using anti-SHISA7 antibody (MBS1753846).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysatesLane 2: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-SHISA7 antigen affinity purified polyclonal antibody (Catalog # MBS1753846) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for SHISA7 at approximately 70KD. The expected band size for SHISA7 is at 70KD. )
Related Product Information for anti-SHISA7 antibody
Shisa7, a single-passing transmembrane protein, localizes at GABAergic inhibitory synapses and interacts with GABA-A receptors. Shisa7 controlled receptor abundance at synapses and sped up the channel deactivation kinetics. Shisa7 also potently enhanced the action of diazepam, a classic benzodiazepine, on GABA-A receptors. Genetic deletion of Shisa7 selectively impaired GABAergic transmission and diminished the effects of diazepam in mic
References
1. Han, W., Li, J., Pelkey, K. A., Pandey, S., Chen, X., Wang, Y. -X., Wu, K., Ge, L., Li, T., Castellano, D., Liu, C., Wu, L. -G., Petralia, R. S., Lynch, J. W., McBain, C. J., Lu, W. Shisa7 is a GABAA receptor auxiliary subunit controlling benzodiazepine actions. Science 366: 246-250, 2019.
2. Hartz, P. A. Personal Communication. Baltimore, Md. 1/30/2017.
3. Pei, J., Grishin, N. V. Unexpected diversity in Shisa-like proteins suggests the importance of their roles as transmembrane adaptors. Cell. Signal. 24: 758-769, 2012.
2. Hartz, P. A. Personal Communication. Baltimore, Md. 1/30/2017.
3. Pei, J., Grishin, N. V. Unexpected diversity in Shisa-like proteins suggests the importance of their roles as transmembrane adaptors. Cell. Signal. 24: 758-769, 2012.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
56,214 Da
NCBI Official Full Name
protein shisa-7
NCBI Official Synonym Full Names
shisa family member 7
NCBI Official Symbol
SHISA7
NCBI Protein Information
protein shisa-7
UniProt Protein Name
Protein shisa-7
Protein Family
UniProt Gene Name
SHISA7
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Product Notes
The SHISA7 shisa7 (Catalog #AAA1753846) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SHISA7 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SHISA7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Mouse, Rat| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the SHISA7 shisa7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHISA7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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