Rabbit SHISA9 Polyclonal Antibody | anti-SHISA9 antibody

SHISA9 Antibody

Cat. Num. AAA540697

• Reactivity: Human, Monkey, Mouse, Rat
• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Synonyms: SHISA9; Polyclonal Antibody; SHISA9 Antibody; CKAMP44; shisa family member 9 antibody; anti-SHISA9 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Form/Format: Affinity Purified Immunoglobulins
• Concentration: 0.72 mg/ml IgG in antibody stabilization buffer (varies by lot)
• Sequence Length: 423

• Applicable Applications for anti-SHISA9 antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Dot Blot: 1:10,000; ELISA: 1:10,000; Western Blot: 1:500
• Immunogen: Synthetic peptide taken within aa acids 350-423 of Human CKAMP44a protein.
• Determinant: N-epitope
• Preparation and Storage: Store at -20°C for long term storage.

Western Blot (WB)

Related Product Information for anti-SHISA9 antibody

Cytosine-knot AMPAR modulating protein 44 (CKAMP44A) is a novel protein that was recently identified as a brain-specific type I transmembrane protein that associates with AMPA receptors in synaptic spines (1). AMPA receptors (AMPARs) mediate fast synaptic transmissions in the vertebrate central nervous system (2). AMPARs are composed of four types of subunits, named as GluR1, GluR2, Glur3, and GluR4, which combine to form tetramers (3, 4). Several AMPAR-interacting proteins have been identified that affect the receptors' subcellular localization, synaptic stabilization, and kinetics, these include transmembrane AMPAR regulatory proteins (TARPs), Sol-1 and cornichons (5-7). CKAMP44A, located on human and mouse chromosome 16, was recently identified as another AMPAR-interacting protein (l). CKAMP44A was shown to modulate short-term plasticity at some excitatory synapses. ln Xenopus Oocytes, expression of CKAMP44A reduced GluR1 and GluR2 mediated steady-state currents, but it did not affect kainite or NMDA receptor-mediated currents (1).

CKAMP44A contains an extracellular N-terminal cysteine-rich motif with eight cysteines that are highly conserved across vertebrate species. Six of these eight cysteine residues might function as stabilizers for the Cys-knot structure of the CKAMP44A (l). RT-PCR analysis from various mouse tissues RNA revealed two splice variants, CKAMP44Aa and CKAMP44Ab, which differ by only 48 bases (1). This CKAMP44A-specific antibody recognizes CKAMP44Aa. Its expression is restricted to the brain; its mRNA is present in most brain structures during embryonic and postnatal development. CKAMP44Aa is approximately a 52kDa protein (432 amino acids).

Product Categories/Family for anti-SHISA9 antibody

Primary Antibodies; Neuroscience; Neurotransmission; SHISA9 Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 290874960
• UniProt Accession #: Q9CZN4; D4NZG2; D4NZG3
• NCBI Official Full Name: cystine-knot AMPAR modulating protein 44a
• UniProt Protein Name: Protein shisa-9
• Protein Family: Protein shisa
• UniProt Gene Name: Shisa9
• UniProt Synonym Gene Names: CKAMP44
• UniProt Entry Name: SHSA9_MOUSE

Uniprot Description

SHISA9: Regulator of short-term neuronal synaptic plasticity in the dentate gyrus. Associates with AMPA receptors (ionotropic glutamate receptors) in synaptic spines and promotes AMPA receptor desensitization at excitatory synapses. Belongs to the shisa family. SHISA9 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: cell junction; cell projection; integral to membrane; ionotropic glutamate receptor complex; membrane; plasma membrane; synapse

Molecular Function: PDZ domain binding

Biological Process: regulation of short-term neuronal synaptic plasticity