Mouse anti-Pig, Human CD58 Antibody | anti-CD58 antibody

Anti-Hu CD58 FITC

Cat. Num. AAA1801266

• Gene Names: CD58; ag3; LFA3; LFA-3
• Reactivity: Pig, Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD58; Anti-Hu CD58 FITC; CD58 molecule; LFA3; AG3; anti-CD58 antibody

• Host: Mouse
• Reactivity: Pig, Human
• Isotype: IgG1
• Clone Number: MEM-63
• Specificity: The antibody MEM-63 reacts with CD58 (LFA-3), a 40-70 kDa extracellular membrane glycoprotein distributed over many tissues, leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts.
• Purity/Purification: The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing Tris buffered saline (TBS) solution with 15 mM sodium azide
• Sequence Length: 248

• Applicable Applications for anti-CD58 antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: QC tested
• Immunogen: NALM-6 human pre-B cell line
• Conjugation: FITC
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.

Related Product Information for anti-CD58 antibody

CD58 (LFA-3) is an immunoglobulin family adhession molecule expressed by both hematopoietic and non-hematopoietic cells (often on antigen presenting cells) and serving as ligand of CD2. This interaction is important for T cell-mediated immunity. CD58 is expressed in transmembrane form and in GPI-anchored form; the later is constitutively associated with protein kinases whereas the transmembrane form activates kinase activity upon triggering. CD58 is a powerful tool for detection of minimal residual disease in acute lymphocytic leukemia, and for evaluation of liver damage related with hepatitis B.

NCBI and Uniprot Product Information

• NCBI GI #: 221316575
• NCBI GeneID: 965
• NCBI Accession #: NP_001138294.1
• NCBI GenBank Nucleotide #: NP_001138294.1
• UniProt Accession #: P19256
• NCBI Official Full Name: lymphocyte function-associated antigen 3 isoform 2
• NCBI Official Synonym Full Names: CD58 molecule
• NCBI Official Symbol: CD58
• NCBI Official Synonym Symbols: ag3; LFA3; LFA-3
• NCBI Protein Information: lymphocyte function-associated antigen 3
• UniProt Protein Name: Lymphocyte function-associated antigen 3
• Protein Family: Lymphocyte function-associated antigen
• UniProt Gene Name: CD58
• UniProt Synonym Gene Names: LFA3; Ag3
• UniProt Entry Name: LFA3_HUMAN

NCBI Description

This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]

Uniprot Description

CD58: Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen- presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: cell surface; membrane; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: heterotypic cell-cell adhesion; cell-cell adhesion; cell adhesion; blood coagulation; leukocyte migration