Western Blot (WB)
(Figure 1: Western blot analysis using NME1 mAb against NME1-hIgGFc transfected HEK293 cell lysate.)
Mouse anti-Human NME1 Antibody | anti-NME1 antibody
NME1 Antibody
IHC: 1:200-1:1000
IF: 1:200-1:1000
FC: 1:200-1:400
ELISA: 1:10000
Western Blot (WB)
(Figure 1: Western blot analysis using NME1 mAb against NME1-hIgGFc transfected HEK293 cell lysate.)
Western Blot (WB)
(Figure 1: Western blot analysis using NME1 mAb against NME1-hIgGFc transfected HEK293 cell lysate.)
Immunohistochemistry (IHC)
(Figure 2: Immunohistochemical analysis of paraffin-embedded human Placenta tissues using anti-NME1 mouse mAb)
Immunohistochemistry (IHC)
(Figure 2: Immunohistochemical analysis of paraffin-embedded human Placenta tissues using anti-NME1 mouse mAb)
Immunofluorescence (IF)
(Figure 3: Immunofluorescence analysis of Hela cells using NME1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.)
Immunofluorescence (IF)
(Figure 3: Immunofluorescence analysis of Hela cells using NME1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.)
NCBI and Uniprot Product Information
NCBI Description
This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]
Uniprot Description
Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.
Research Articles on NME1
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Product Notes
The NME1 nme1 (Catalog #AAA850299) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NME1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NME1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:200-1:1000 IF: 1:200-1:1000 FC: 1:200-1:400 ELISA: 1:10000. Researchers should empirically determine the suitability of the NME1 nme1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NME1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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