Mouse anti-Human LIME1 Antibody | anti-LIME1 antibody

Anti-LIME1 Purified

Cat. Num. AAA1800864

• Gene Names: LIME1; LIME; dJ583P15.4
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by Protein-A Affinity Chromatography
• Synonyms: LIME1; Anti-LIME1 Purified; Lck interacting transmembrane adaptor 1; LITA1; anti-LIME1 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG2a
• Clone Number: LIME-10
• Specificity: The antibody LIME-10 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.
• Purity/Purification: Purified by Protein-A Affinity Chromatography
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 295

• Applicable Applications for anti-LIME1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Immunogen: COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated to keyhole limpet hemocyanin.
• Preparation and Storage: Store at 2-8 degree C.

Western Blot (WB)

(Western blotting analysis (reducing conditions) of human LIME using anti-human LIME (LIME-10).Lane 1: J77 cell line transfected with LIMELane 2: non-transfected J77 cell line (cells are essentialy devoid of endogenous LIME expression))

Related Product Information for anti-LIME1 antibody

LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.

NCBI and Uniprot Product Information

• NCBI GI #: 157388906
• NCBI GeneID: 54923
• NCBI Accession #: NP_060276.2
• NCBI GenBank Nucleotide #: NP_060276.2
• UniProt Accession #: Q9H400
• NCBI Official Full Name: lck-interacting transmembrane adapter 1 isoform 1
• NCBI Official Synonym Full Names: Lck interacting transmembrane adaptor 1
• NCBI Official Symbol: LIME1
• NCBI Official Synonym Symbols: LIME; dJ583P15.4
• NCBI Protein Information: lck-interacting transmembrane adapter 1
• UniProt Protein Name: Lck-interacting transmembrane adapter 1
• Protein Family: Lck-interacting transmembrane adapter
• UniProt Gene Name: LIME1
• UniProt Synonym Gene Names: LIME; Lck-interacting membrane protein
• UniProt Entry Name: LIME1_HUMAN

NCBI Description

This gene encodes a transmembrane adaptor protein that links the T and B-cell receptor stimulation to downstream signaling pathways via its association with the Src family kinases Lck and Lyn, respectively. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Mar 2015]

Uniprot Description

LIME1: Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T- cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: extracellular space; plasma membrane; integral to membrane

Biological Process: B cell receptor signaling pathway; T cell receptor signaling pathway