Rabbit anti-Mouse IL-4 Polyclonal Antibody | anti-IL4 antibody

Anti-IL-4 Antibody, Rabbit Polyclonal

Cat. Num. AAA8112303

• Gene Names: Il4; Il-4; BSF-1
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: IL-4; Polyclonal Antibody; Anti-IL-4 Antibody; Rabbit Polyclonal; IL-4 Antibody; Rabbit PAb; Antigen Affinity Purified; interleukin 4; Anti-BSF-1 Antibody; Anti-Il-4 Antibody; anti-IL4 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse IL-4
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-IL4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000
• Immunogen: Recombinant Mouse IL-4 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse IL-4 (rM IL-4). IL-4 specific IgG was purified by Mouse IL-4 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-IL-4 rabbit polyclonal antibody at 1:500 dilution Lane A: mouse spleen Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:17 kDa Observed band size:21 kDa)

Related Product Information for anti-IL4 antibody

Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4/IL-13 family. Interleukin-4/IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4/IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibits in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.

Product Categories/Family for anti-IL4 antibody

Common G-Chain signaling pathway

References

Grunewald SM. et al., 1998, J Immunol. 160 (8): 4004-9. Susanne M. et al, 1997, THE JOURNAL OF BIOLOGICAL CHEMISTRY. 272 (3): 1480-3. Nishikubo K. et al., 2003, Gene Ther. 10 (26): 2119-25.

NCBI and Uniprot Product Information

• NCBI GI #: 10946584
• NCBI GeneID: 16189
• NCBI Accession #: NP_067258.1
• NCBI GenBank Nucleotide #: NM_021283.2
• UniProt Accession #: P07750
• Molecular Weight: 15,834 Da
• NCBI Official Full Name: interleukin-4
• NCBI Official Synonym Full Names: interleukin 4
• NCBI Official Symbol: Il4
• NCBI Official Synonym Symbols: Il-4; BSF-1
• NCBI Protein Information: interleukin-4; B-cell IgG differentiation factor; B-cell growth factor 1; B-cell stimulatory factor 1; IGG1 induction factor; lymphocyte stimulatory factor 1
• UniProt Protein Name: Interleukin-4
• Protein Family: Interleukin
• UniProt Gene Name: Il4
• UniProt Synonym Gene Names: Il-4; IL-4; BSF-1
• UniProt Entry Name: IL4_MOUSE

Uniprot Description

IL4: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the IL-4/IL-13 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Secreted, signal peptide; Motility/polarity/chemotaxis; Cytokine; Secreted

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: growth factor activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity; interleukin-4 receptor binding

Biological Process: negative regulation of osteoclast differentiation; positive regulation of isotype switching to IgG isotypes; positive regulation of transcription, DNA-dependent; microglial cell activation; regulation of proton transport; positive regulation of activated T cell proliferation; positive regulation of interleukin-10 production; positive regulation of isotype switching to IgE isotypes; B cell costimulation; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of T cell proliferation; positive regulation of interleukin-13 production; negative regulation of macrophage activation; cholesterol metabolic process; negative regulation of chronic inflammatory response; regulation of immune response; B cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of acute inflammatory response; positive regulation of mast cell degranulation; defense response to protozoan; positive regulation of immunoglobulin production; T-helper 1 cell lineage commitment; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of chemokine biosynthetic process; positive regulation of T cell differentiation; positive regulation of tyrosine phosphorylation of Stat5 protein; innate immune response in mucosa; positive regulation of B cell activation; regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; immune response; positive regulation of protein amino acid phosphorylation; T-helper 2 cell differentiation; positive regulation of defense response to virus by host; negative regulation of transcription, DNA-dependent; negative regulation of T cell activation