Mouse anti-Human JMJD2A Antibody | anti-JMJD2A antibody

AbFlex JMJD2A antibody (rAb)

Cat. Num. AAA389449

• Gene Names: KDM4A; JMJD2; JHDM3A; JMJD2A; TDRD14A
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Protein A Chromatography
• Synonyms: JMJD2A; AbFlex JMJD2A antibody (rAb); KDM4A; JMJD2; JHDM3A; TDRD14A; anti-JMJD2A antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG2a
• Purity/Purification: Protein A Chromatography
• Form/Format: Purified IgG in 200mM Hepes pH7.5, 100mM NaCl, 50mM NaOAc, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-JMJD2A antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications Validated:; WB: 1-2ug/ml; Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
• Antibody Type: Recombinant Antibody
• Immunogen: This JMJD2A antibody was raised against a recombinant protein corresponding to amino acids 586-674 of human JMJD2A.
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

Western Blot (WB)

(AbFlex JMJD2A antibody (rAb) tested by Western blot. HeLa cell nuclear extract (20 ug) probed with AbFlex JMJD2A antibody (rAb) at a dilution of 2 ug/ml.)

Testing Data

(AbFlex JMJD2A antibody (rAb) tested by Luminex bead-based specificity analysis. Luminex bead-based specificity analysis was used to confirm the specificity of AbFlexTM JMJD2A antibody (rAb) antibody for JMJD2A. Various proteins were conjugated to MagPlex Luminex beads and incubated with various amounts of AbFlexTM JMJD2A antibody (rAb). Protein-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument. Luminex is a registered trademark of Luminex Corporation.)

Related Product Information for anti-JMJD2A antibody

Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex JMJD2A antibody (rAb) was raised in a Mouse host. It has been validated for use in ELISA and Western blot; it has been shown to react with Human samples.

Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex JMJD2A antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. JMJD2A (KDM4A, JHDM3A) is a member of the Jmj-containing (Jumonji) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. JMJD2A specifically demethylates histone H3 dimethyl lysine 9 (H3K9me2), trimethyl lysine 9, (H3K9me3), dimethyl lysine 36 (H3K36me2) and trimethyl lysine 36 (H3K36me3). It also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1. JMJD2A contains JmjC and JmjN domains, two PHD fingers and two tudor domains.

Product Categories/Family for anti-JMJD2A antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 98986459
• NCBI GeneID: 9682
• NCBI Accession #: NP_055478; AB_2793788
• NCBI GenBank Nucleotide #: NM_014663
• UniProt Accession #: O75164; Q5VVB1
• Molecular Weight: 54,697 Da
• NCBI Official Full Name: lysine-specific demethylase 4A
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 4A
• NCBI Official Symbol: KDM4A
• NCBI Official Synonym Symbols: JMJD2; JHDM3A; JMJD2A; TDRD14A
• NCBI Protein Information: lysine-specific demethylase 4A; jmjC domain-containing histone demethylation protein 3A; jumonji C domain-containing histone demethylase 3A; jumonji domain containing 2; jumonji domain containing 2A; jumonji domain-containing protein 2A; tudor domain cont
• UniProt Protein Name: Lysine-specific demethylase 4A
• UniProt Gene Name: KDM4A
• UniProt Synonym Gene Names: JHDM3A; JMJD2; JMJD2A; KIAA0677
• UniProt Entry Name: KDM4A_HUMAN

Uniprot Description

JMJD2A: Histone demethylase that specifically demethylates 'Lys- 9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. Interacts with histone deacetylase proteins HDAC1, HDAC2 and HDAC3. Interacts with RB and NCOR1. Interacts with HTLV-1 Tax protein. Ubiquitous. Belongs to the JHDM3 histone demethylase family.

Protein type: Transcription, coactivator/corepressor; Demethylase; EC 1.14.11.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; zinc ion binding; ubiquitin protein ligase binding; histone demethylase activity (H3-K36 specific); methylated histone residue binding

Biological Process: cardiac muscle hypertrophy; histone demethylation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; viral reproduction; negative regulation of transcription, DNA-dependent