Rabbit anti-Human JMJD2A Polyclonal Antibody | anti-JMJD2A antibody

JMJD2A, NT (JMJD2A, JHDM3A, JMJD2, KIAA0677, Lysine-specific Demethylase 4A, JmjC Domain-containing Histone Demethylation Protein 3A, Jumonji domain-Containing Protein 2A, KDM4A) (AP)

Cat. Num. AAA6483865

• Gene Names: KDM4A; JMJD2; JHDM3A; JMJD2A; TDRD14A
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: JMJD2A; Polyclonal Antibody; NT (JMJD2A; JHDM3A; JMJD2; KIAA0677; Lysine-specific Demethylase 4A; JmjC Domain-containing Histone Demethylation Protein 3A; Jumonji domain-Containing Protein 2A; KDM4A) (AP); anti-JMJD2A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human JMJD2A.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-JMJD2A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human JMJD2A (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of JMJD2A(arrow) using rabbit polyclonal JMJD2A (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the JMJD2A gene (Lane 2) (Origene Technologies).)

Product Categories/Family for anti-JMJD2A antibody

Antibodies; Enzymes; Demethylase

References

1. Gray S.G., Iglesias A.H.J. Biol. Chem. 280:28507-28518(2005). 2. Zhang D., Yoon H.-G.Mol. Cell. Biol. 25:6404-6414(2005). 3. Whetstine J.R., Nottke A.Cell 125:467-481(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 98986459
• NCBI GeneID: 9682
• NCBI Accession #: NP_055478
• NCBI GenBank Nucleotide #: NM_014663
• UniProt Accession #: O75164; Q5VVB1
• Molecular Weight: 54,697 Da
• NCBI Official Full Name: lysine-specific demethylase 4A
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 4A
• NCBI Official Symbol: KDM4A
• NCBI Official Synonym Symbols: JMJD2; JHDM3A; JMJD2A; TDRD14A
• NCBI Protein Information: lysine-specific demethylase 4A; jmjC domain-containing histone demethylation protein 3A; jumonji C domain-containing histone demethylase 3A; jumonji domain containing 2; jumonji domain containing 2A; jumonji domain-containing protein 2A; tudor domain cont
• UniProt Protein Name: Lysine-specific demethylase 4A
• UniProt Gene Name: KDM4A
• UniProt Synonym Gene Names: JHDM3A; JMJD2; JMJD2A; KIAA0677
• UniProt Entry Name: KDM4A_HUMAN

Uniprot Description

JMJD2A: Histone demethylase that specifically demethylates 'Lys- 9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. Interacts with histone deacetylase proteins HDAC1, HDAC2 and HDAC3. Interacts with RB and NCOR1. Interacts with HTLV-1 Tax protein. Ubiquitous. Belongs to the JHDM3 histone demethylase family.

Protein type: Transcription, coactivator/corepressor; Demethylase; EC 1.14.11.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; zinc ion binding; ubiquitin protein ligase binding; histone demethylase activity (H3-K36 specific); methylated histone residue binding

Biological Process: cardiac muscle hypertrophy; histone demethylation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; viral reproduction; negative regulation of transcription, DNA-dependent