Mouse anti-Human Hif-1a Antibody | anti-Hif-1a antibody

Anti-Hif-1a Purified (with Antibiotics)

Cat. Num. AAA666933

• Gene Names: HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
• Reactivity: Human
• Synonyms: Hif-1a; Anti-Hif-1a Purified (with Antibiotics); Anti-Human Hif-1a; Hypoxia Inducing Factor; Hif1a; anti-Hif-1a antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1k
• Clone Number: ANC10G3
• Sequence Length: 826

• Immunogen: Recombinant human Hif1a

Related Product Information for anti-Hif-1a antibody

Hypoxia Inducible Factor 1 alpha (Hif-1a) is a transcription factor which accumulates in the nucleus of cells in most tissues which are subjected to hypoxic conditions. There it stabilizes by associating with Hif-1 beta (ARNT).
Clone ANC10G3 binds to recombinant human Hif-1a in EIA, as well as native HIf1a present in nuclei of fixed permeabolized Nalm-6 cells cultured overnight with CoCl2 to simulate hypoxia. It interferes with binding of anti-Hif1a clone 546-16, suggesting that their epitopes overlap.

Product Categories/Family for anti-Hif-1a antibody

Antibodies; Conjugates

References

1) GL Wang, GL Song, et al. (1993) PNAS 90: 4304

NCBI and Uniprot Product Information

• NCBI GI #: 4504385
• NCBI GeneID: 3091
• NCBI Accession #: NP_001521.1
• NCBI GenBank Nucleotide #: NM_001530.4
• UniProt Accession #: Q16665; Q53XP6; Q96PT9; Q9UPB1; C0LZJ3
• Molecular Weight: 82,746 Da
• NCBI Official Full Name: hypoxia-inducible factor 1-alpha isoform 1
• NCBI Official Synonym Full Names: hypoxia inducible factor 1 subunit alpha
• NCBI Official Symbol: HIF1A
• NCBI Official Synonym Symbols: HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
• NCBI Protein Information: hypoxia-inducible factor 1-alpha
• UniProt Protein Name: Hypoxia-inducible factor 1-alpha
• UniProt Gene Name: HIF1A
• UniProt Synonym Gene Names: BHLHE78; MOP11 PublicationManual assertion based on opinion iniRef.2; HIF-1-alpha1 PublicationManual assertion based on opinion iniRef.1; HIF1-alpha1 PublicationManual assertion based on opinion iniRef.1; bHLHe78

NCBI Description

This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.