Testing Data
(We used U937 cells 3 days after passaging. Half were activated with IFN-gamma 100 ng/ml for 20 hours and half were used as control. Cell concentration at the beginning of activation was 2.105
cells/ml. Brefaldin A was used as an intracellular blocking agent at 1ug/ml, in contact with the cells for 4 to 6 hours. The black line on the graph represents the IgG2b isotype control (B-E4), the green line shows B-C50 with U937 non-activated cells and the yellow line represents activated U937 cells marked with B-C50 (10ul per test for 5.105 cells))
Mouse anti-Human IP-10 Monoclonal Antibody | anti-IP-10 antibody
Mouse Anti-Human IP-10 Monoclonal Antibody, FITC Conjugated Clone B-C50
Testing Data
(We used U937 cells 3 days after passaging. Half were activated with IFN-gamma 100 ng/ml for 20 hours and half were used as control. Cell concentration at the beginning of activation was 2.105 cells/ml. Brefaldin A was used as an intracellular blocking agent at 1ug/ml, in contact with the cells for 4 to 6 hours. The black line on the graph represents the IgG2b isotype control (B-E4), the green line shows B-C50 with U937 non-activated cells and the yellow line represents activated U937 cells marked with B-C50 (10ul per test for 5.105 cells))
Testing Data
(We used U937 cells 3 days after passaging. Half were activated with IFN-gamma 100 ng/ml for 20 hours and half were used as control. Cell concentration at the beginning of activation was 2.105
cells/ml. Brefaldin A was used as an intracellular blocking agent at 1ug/ml, in contact with the cells for 4 to 6 hours. The black line on the graph represents the IgG2b isotype control (B-E4), the green line shows B-C50 with U937 non-activated cells and the yellow line represents activated U937 cells marked with B-C50 (10ul per test for 5.105 cells))
NCBI and Uniprot Product Information
Uniprot Description
CXCL10: Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. Belongs to the intercrine alpha (chemokine CxC) family.
Protein type: Secreted; Chemokine; Secreted, signal peptide; Motility/polarity/chemotaxis
Cellular Component: extracellular space; cell; extracellular region; external side of plasma membrane
Molecular Function: heparin binding; CXCR3 chemokine receptor binding; chemokine activity; cytokine activity
Biological Process: positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of cAMP metabolic process; chemotaxis; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; positive regulation of cell proliferation; immune response; positive regulation of release of sequestered calcium ion into cytosol; inflammatory response; negative regulation of myoblast differentiation; defense response to virus; positive regulation of cell migration
Research Articles on IP-10
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Product Notes
The IP-10 cxcl10 (Catalog #AAA335101) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human IP-10 Monoclonal Antibody, FITC Conjugated Clone B-C50 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IP-10 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Working Dilution: Use 10 mul to label 106 cells or 100 mul of whole blood. Researchers should empirically determine the suitability of the IP-10 cxcl10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IP-10, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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