Hif-1a recombinant protein

Hif-1a, 530-826aa, Human, E Coli

Cat. Num. AAA203066

• Gene Names: HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: Hif-1a; 530-826aa; Human; E Coli; Hypoxia-inducible factor-1a; HIF1A; MOP1; Hif-1 alpha (530-826 aa); HIF-1 alpha; HIF1 alpha; ARNT-interacting protein; Member of PAS protein 1; Basic-helix-loop-helix-PAS protein MOP1; Hypoxia-inducible factor 1 alpha; Hypoxia-inducible factor-1a Basic helix loop helix PAS protein MOP1; ARNT interacting protein; bHLHe78; HIF 1A; HIF 1alpha; HIF1 A; Hypoxia inducible factor 1 alpha; Hypoxia inducible factor 1 alpha isoform I.3; Hypoxia inducible factor 1 alpha subunit; Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor; Member of PAS superfamily 1; Member of the PAS Superfamily 1; MOP 1; PASD 8; PASD8; ; Hif-1a recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl pH7.5, 1mMDTT
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MEFKLELVEK LFAEDTEAKN PFSTQDTDLD LEMLAPYIPM DDDFQLRSFD QLSPLESSSA SPESASPQST VTVFQQTQIQ EPTANATTTT ATTDELKTVT KDRMEDIKIL IASPSPTHIH KETTSATSSP YRDTQSRTAS PNRAGKGVIE QTEKSHPRSP NVLSVALSQR TTVPEEELNP KILALQNAQR KRKMEHDGSL FQAVGIGTLL QQPDDHAATT SLSWKRVKGC KSSEQNGMEQ KTIILIPSDL ACRLLGQSMD ESGLPQLTSY DCEVNAPIQG SRNLLQGEEL LRALDQVN
• Sequence Length: 826

• Applicable Applications for Hif-1a recombinant protein: SDS-PAGE
• Antigen Species: Human
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Hif-1a recombinant protein

Hypoxia-inducible factor-1 (HIF-1), identified as one of the transcription factors, has been found to play an essential role in oxygen homeostasis. HIF-1 is a heterodimer composed of HIF-1beta subunit and one of three subunits (Hif-1alpha, Hif-2 or Hif-3). The activation of Hif-1 is closely associated with a variety of tumors and oncogenic pathways. Hif-1alpha consists of DNA binding domain (DBD domain), Dimerization domain and C-terminla regulatiory domains, including two transactivation domains (TAD), an oxygen-dependent degradation (ODD) domain, and inhibitory domains. Hif-1 alpha (530-826 residues) contains two TAD and inhibitory domain. Recombinant Hif-1 alpha (530-826 residues) was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for Hif-1a recombinant protein

Cell Cycle

References

Okuyama H. et al., (2006) J. Biol Chem. 281 (22):15554-63; Berra E. et al., (2006) EMBO.7 (1):41-5;

NCBI and Uniprot Product Information

• NCBI GI #: 4504385
• NCBI GeneID: 3091
• NCBI Accession #: NP_001521
• NCBI GenBank Nucleotide #: NM_001530.3
• UniProt Accession #: Q16665; Q53XP6; Q96PT9; Q9UPB1; C0LZJ3
• Molecular Weight: 32.8kDa (298aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
• NCBI Official Full Name: hypoxia-inducible factor 1-alpha isoform 1
• NCBI Official Synonym Full Names: hypoxia inducible factor 1 alpha subunit
• NCBI Official Symbol: HIF1A
• NCBI Official Synonym Symbols: HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
• NCBI Protein Information: hypoxia-inducible factor 1-alpha
• UniProt Protein Name: Hypoxia-inducible factor 1-alpha
• UniProt Gene Name: HIF1A
• UniProt Synonym Gene Names: bHLHe78

NCBI Description

This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.