Mouse anti-Human GLUT-1 Antibody | anti-GLUT-1 antibody

GLUT-1 (Tumor Progression and Mesothelioma Marker)

Cat. Num. AAA4381252

• Gene Names: SLC2A1; CSE; PED; DYT9; GLUT; DYT17; DYT18; EIG12; GLUT1; HTLVR; GLUT-1; SDCHCN; GLUT1DS
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: GLUT-1; GLUT-1 (Tumor Progression and Mesothelioma Marker); Erythrocyte/hepatoma glucose transporter; Glucose transporter type-1; GLUT1; GLUT1DS; GLUTB; GT1; GTG1; Gtg3; HepG2 glucose transporter; PED; RATGTG1; Solute carrier family 2; facilitated glucose transporter member 1 (SLC2A1); anti-GLUT-1 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1, kappa
• Clone Number: rGLUT1/2476
• Specificity: Recognizes a protein of 55kDa, which is identified as GLUT-1. Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. There are many types of glucose transport carrier proteins, designated as Glut-1 to Glut-12. Glut-1 is a major glucose transporter in the mammalian blood-brain barrier. It is expressed in high density on the membranes of human erythrocytes and the brain capillaries that comprise the blood-brain barrier. Glut-1 is expressed at variable levels in many human tissues. Overexpression of Glut-1 has been linked to tumor progression or poor survival of patients with carcinomas of the colon, breast, cervical, lung, bladder and mesothelioma. Glut-1 is a sensitive and specific marker for the differentiation of malignant mesothelioma (positive) from reactive mesothelium (negative).
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 492

• Applicable Applications for anti-GLUT-1 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin
• Application Notes: ELISA (For coating use Ab at 1-2ug/ml order Ab without BSA); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: K562, A431, MDA-MB-231 cells or erythrocytes. Mesothelioma or breast, colon and ovarian carcinoma.
• Immunogen: Recombinant fragment of human GLUT1 protein (around aa 203-305) (exact sequence is proprietary)
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 1p34.2
• Antibody Type: Recombinant Mouse Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE Analysis Purified GLUT-1 Recombinant Mouse Monoclonal Antibody (rGLUT1/2476). Confirmation of Purity and Integrity of Antibody.)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Bladder stained with GLUT-1 Mouse Monoclonal Antibody (rGLUT1/2476).)

NCBI and Uniprot Product Information

• NCBI GI #: 166795299
• NCBI GeneID: 6513
• NCBI Accession #: NP_006507.2
• NCBI GenBank Nucleotide #: NP_006507.2
• UniProt Accession #: P11166
• Molecular Weight: 55kDa
• NCBI Official Full Name: solute carrier family 2, facilitated glucose transporter member 1
• NCBI Official Synonym Full Names: solute carrier family 2 member 1
• NCBI Official Symbol: SLC2A1
• NCBI Official Synonym Symbols: CSE; PED; DYT9; GLUT; DYT17; DYT18; EIG12; GLUT1; HTLVR; GLUT-1; SDCHCN; GLUT1DS
• NCBI Protein Information: solute carrier family 2, facilitated glucose transporter member 1
• UniProt Protein Name: Solute carrier family 2, facilitated glucose transporter member 1
• UniProt Gene Name: SLC2A1
• UniProt Synonym Gene Names: GLUT1; GLUT-1
• UniProt Entry Name: GTR1_HUMAN

NCBI Description

This gene encodes a major glucose transporter in the mammalian blood-brain barrier. The encoded protein is found primarily in the cell membrane and on the cell surface, where it can also function as a receptor for human T-cell leukemia virus (HTLV) I and II. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. [provided by RefSeq, Apr 2013]

Uniprot Description

GLUT1: an integral membrane protein that plays an important role in the glycolytic pathway by serving as a uniporter for glucose. One of 13 members of the human equilibrative glucose transport protein family. Transports a wide range of aldoses, including both pentoses and hexoses, and dehydroascorbic acid. Shown to transport water against an osmotic gradient. A receptor for the Human T-cell Leukemia virus (HTLV). Plays a role in the constitutive or basal uptake of glucose. Expressed at highest levels in proliferating cells of the early developing embryo, cells forming the blood tissue barriers, in human erythrocytes, astrocytes and in cardiac muscle. GLUT1 and GLUT3 are both essential for normal embryonic development. Is practically the only member of the GLUT family expressed on human red blood cells, where it comprises 10 - 20% of the integral membrane protein content. Several glycolytic proteins including the transporters GLUT1 and GLUT3, as well as multiple enzymes including HK2, PFKL, LDHA, ALDOA, ALDOC, PGK1, ENO1, PKM2, CA9 and PFKFB3 are induced in cancer cells by HIF-1 alpha. Polyps from Peutz-Jeghers patients exhibit up-regulated mTORC1 signaling, HIF-1alpha, and GLUT1 levels. Defects in GLUT1 are the cause of autosomal dominant GLUT1 deficiency syndrome, a blood-brain barrier glucose transport defect characterized by infantile seizures, delayed development, and acquired microcephaly. Defects also cause dystonia type 18, an exercise-induced paroxysmal dystonia/dyskinesia. Cytochalasin B binds to its inner surface, inhibiting its glucose transport activity with an IC50 of 0.44 uM.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter, SLC family; Transporter

Chromosomal Location of Human Ortholog: 1p34.2

Cellular Component: cortical actin cytoskeleton; membrane; basolateral plasma membrane; integral to plasma membrane; melanosome; plasma membrane; female pronucleus; midbody; caveola; intercellular junction; cytosol

Molecular Function: identical protein binding; D-glucose transmembrane transporter activity; xenobiotic transporter activity; protein binding; protein self-association; dehydroascorbic acid transporter activity; glucose transmembrane transporter activity; kinase binding

Biological Process: cellular response to glucose starvation; vitamin metabolic process; hexose transport; carbohydrate metabolic process; L-ascorbic acid metabolic process; energy reserve metabolic process; pathogenesis; xenobiotic transport; protein complex assembly; glucose transport; response to osmotic stress; transmembrane transport; regulation of insulin secretion; water-soluble vitamin metabolic process

Disease: Epilepsy, Idiopathic Generalized, Susceptibility To, 12; Dystonia 9; Glut1 Deficiency Syndrome 1; Glut1 Deficiency Syndrome 2