Goat anti-Human CLEC1B Polyclonal Antibody | anti-CLEC1B antibody

CLEC1B (C-type Lectin Domain Family 1 Member B, C-type Lectin-like Receptor 2, CLEC-2, CLEC2, UNQ721/PRO1384)

Cat. Num. AAA646846

• Gene Names: CLEC1B; CLEC2; CLEC2B; PRO1384; QDED721; 1810061I13Rik
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: CLEC1B; Polyclonal Antibody; CLEC1B (C-type Lectin Domain Family 1 Member B; C-type Lectin-like Receptor 2; CLEC-2; CLEC2; UNQ721/PRO1384); Anti -CLEC1B (C-type Lectin Domain Family 1 Member B; anti-CLEC1B antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human CLEC1B.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-CLEC1B antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1000; Western Blot: 1-3ug/ml, observed in human bone marrow and kidney lysates on ~28kD bands
• Immunogen: Synthetic peptide corresponding to KGHKCSPCDTNWRY, from human CLEC1B, at the internal region of the protein (NP_001092901.1, NP_057593.3).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

((1ug/ml) staining of human kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-CLEC1B antibody

Acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (Syk) and initiation of downstream tyrosine phosphorylation events and activation of PLC-gamma-2. Acts as an attachment factor for human immunodeficiency virus type 1 (HIV-1) and facilitates its capture by platelets.

Product Categories/Family for anti-CLEC1B antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 20810586
• NCBI GeneID: 51266
• UniProt Accession #: Q9P126; Q6UWX7; Q8NHR6
• Molecular Weight: 26,596 Da
• NCBI Official Full Name: CLEC1B protein
• NCBI Official Synonym Full Names: C-type lectin domain family 1, member B
• NCBI Official Symbol: CLEC1B
• NCBI Official Synonym Symbols: CLEC2; CLEC2B; PRO1384; QDED721; 1810061I13Rik
• NCBI Protein Information: C-type lectin domain family 1 member B; CLEC-2; C-type lectin-like receptor 2; C-type lectin-like receptor-2
• UniProt Protein Name: C-type lectin domain family 1 member B
• Protein Family: C-type lectin domain family
• UniProt Gene Name: CLEC1B
• UniProt Synonym Gene Names: CLEC2; CLEC-2
• UniProt Entry Name: CLC1B_HUMAN

NCBI Description

Natural killer (NK) cells express multiple calcium-dependent (C-type) lectin-like receptors, such as CD94 (KLRD1; MIM 602894) and NKG2D (KLRC4; MIM 602893), that interact with major histocompatibility complex class I molecules and either inhibit or activate cytotoxicity and cytokine secretion. CLEC2 is a C-type lectin-like receptor expressed in myeloid cells and NK cells (Colonna et al., 2000 [PubMed 10671229]).[supplied by OMIM, Jan 2011]

Uniprot Description

Function: Acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (Syk) and initiation of downstream tyrosine phosphorylation events and activation of PLC-gamma-2. Acts as an attachment factor for human immunodeficiency virus type 1 (HIV-1) and facilitates its capture by platelets. Ref.2 Ref.6

Subunit structure: Homodimer. Interacts (via cytoplasmic domain) with GNB2L1/RACK1; promotes CLEC1B ubiquitination and proteasome-mediated degradation. Interacts (dimer) with SYK (via SH2 domains). Ref.7 Ref.8 Ref.9 Ref.10

Subcellular location: Membrane; Single-pass type II membrane protein

Probable.

Tissue specificity: Expressed preferentially in the liver. Also expressed in immune cells of myeloid origin and on the surface of platelets. Ref.1 Ref.2 Ref.6

Post-translational modification: Glycosylated. Ref.6Phosphorylated on tyrosine residue in response to rhodocytin binding. Ref.6

Sequence similarities: Contains 1 C-type lectin domain.