Mouse X-Box-Binding Protein 1 (XBP1) ELISA Kit | XBP1 elisa kit

Mouse X-Box-Binding Protein 1 (XBP1) ELISA Kit

Cat. Num. AAA9716615

• Gene Names: Xbp1; TREB5; XBP-1; TREB-5; D11Ertd39e
• Reactivity: Mouse
• Synonyms: X-Box-Binding Protein 1 (XBP1); Mouse X-Box-Binding Protein 1 (XBP1) ELISA Kit; XBP1; TREB5; XBP2; XBP1 elisa kit

• Reactivity: Mouse
• Specificity: Mouse X-box-binding protein 1 (XBP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse XBP1. No significant cross-reactivity or interference between Mouse XBP1 and analogues was observed.
• Sequence Length: 371

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse X-box-binding protein 1 (XBP1) Standard Curve.)

Related Product Information for XBP1 elisa kit

Description: This Mouse X-box-binding protein 1 (XBP1) ELISA Kit employs a two-site sandwich ELISA to quantitate XBP1 in samples. An antibody specific for XBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any XBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: X-box binding protein 1is a a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor.

NCBI and Uniprot Product Information

• NCBI GI #: 411147451
• NCBI GeneID: 22433
• NCBI Accession #: NP_001258659.1
• NCBI GenBank Nucleotide #: NP_001258659.1
• UniProt Accession #: O35426
• NCBI Official Full Name: X-box-binding protein 1 isoform XBP1(S)
• NCBI Official Synonym Full Names: X-box binding protein 1
• NCBI Official Symbol: Xbp1
• NCBI Official Synonym Symbols: TREB5; XBP-1; TREB-5; D11Ertd39e
• NCBI Protein Information: X-box-binding protein 1
• UniProt Protein Name: X-box-binding protein 1
• Protein Family: X-box-binding protein
• UniProt Gene Name: Xbp1
• UniProt Synonym Gene Names: TREB-5Curated

Uniprot Description

XBP1: a transcription factor essential for hepatocyte growth, the differentiation of plasma cells, immunoglobulin secretion, and the unfolded protein response (UPR). XBP1 mRNA is spliced by IRE1 during the UPR to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. Only the spliced form of XBP1 can activate the UPR efficiently. Activates UPR target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I. Up-regulated by ATF6 via direct binding to the ERSE in response to endoplasmic reticulum stress. Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7). Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature. Two human isoforms are produced by alternative splicing. Isoform 1 is also known as XBP-1U. Isoform 2, also known as XBP-1S, is produced by IRE1 in response to endoplasmic reticulum stress. IRE1 cleaves a 26-bp fragment causing a frameshift of the mRNA transcript.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 11 A1|11 3.61 cM

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; integral component of endoplasmic reticulum membrane; integral component of membrane; membrane; nucleus

Molecular Function: chromatin DNA binding; DNA binding; DNA binding transcription factor activity; histone deacetylase binding; identical protein binding; protease binding; protein heterodimerization activity; protein kinase binding; sequence-specific DNA binding; ubiquitin protein ligase binding

Biological Process: angiogenesis; apoptosis; autophagy; cell differentiation; cell growth; cellular response to glucose starvation; cellular response to insulin stimulus; cellular response to nutrient; cholesterol homeostasis; endoplasmic reticulum unfolded protein response; endothelial cell proliferation; epithelial cell maturation; exocrine pancreas development; fatty acid homeostasis; glucose homeostasis; lipid metabolic process; liver development; multicellular organism development; muscle organ development; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; neuron development; phosphoinositide 3-kinase cascade; positive regulation of angiogenesis; positive regulation of autophagy; positive regulation of B cell differentiation; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of fat cell differentiation; positive regulation of histone methylation; positive regulation of immunoglobulin production; positive regulation of immunoglobulin secretion; positive regulation of MHC class II biosynthetic process; positive regulation of protein phosphorylation; positive regulation of T cell differentiation; positive regulation of TOR signaling pathway; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription of target genes involved in unfolded protein response; protein destabilization; protein transport; regulation of autophagy; regulation of protein stability; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; response to drug; response to electrical stimulus; response to unfolded protein; serotonin secretion, neurotransmission; sterol homeostasis; transcription from RNA polymerase II promoter; transcription, DNA-dependent; transport; ubiquitin-dependent protein catabolic process; vascular endothelial growth factor receptor signaling pathway