Rabbit anti-Mouse XBP1 Polyclonal Antibody | anti-XBP1 antibody

XBP1 Antibody - middle region

Cat. Num. AAA3223585

• Gene Names: Xbp1; TREB5; XBP-1; TREB-5; D11Ertd39e
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: XBP1; Polyclonal Antibody; XBP1 Antibody - middle region; anti-XBP1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Purity/Purification: Affinity purified
• Form/Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Sequence: Synthetic peptide located within the following region: PRAAGSEASGTPQARKRQRLTHLSPEEKALRRKLKNRVAAQTARDRKKAR
• Sequence Length: 371

• Applicable Applications for anti-XBP1 antibody: Western Blot (WB)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of mouse XBP1
• Preparation and Storage: For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Host: RabbitTarget Name: XBP1Sample Tissue: Mouse Heart lysatesAntibody Dilution: 1ug/ml)

Related Product Information for anti-XBP1 antibody

Functions as a transcription factor during endoplasmic reticulum stress by regulating the unfolded protein response (UPR). Required for cardiac myogenesis and hepatogenesis during embryonic development and the development of secretory tissues such as exocrine pancreas and salivary gland. Involved in differentiation of B lymphocytes to plasma cells and production of immunoglobulins. Modulates the cellular response to ER stress in a PIK3R-dependent manner. Binds to the cis-acting X box present in the promoter regions of major histocompatibility complex class II genes (By similarity). Involved in VEGF-induced endothelial cell (EC) proliferation and retinal blood vessel formation during embryonic development but also for angiogenesis in adult tissues under ischemic conditions. Functions also as a major regulator of the UPR in obesity-induced insulin resistance and type 2 diabetes for the management of obesity and diabetes prevention.

Product Categories/Family for anti-XBP1 antibody

Polyclonal; Transcription Factor; Transcription Regulation; Lymphocyte Signaling; Cardiovascular; Developmental Biology; Immunology; Disease Related

NCBI and Uniprot Product Information

• NCBI GI #: 411147451
• NCBI GeneID: 22433
• NCBI Accession #: NP_001258659.1
• NCBI GenBank Nucleotide #: NM_001271730.1
• UniProt Accession #: O35426
• Molecular Weight: 40 kDa
• NCBI Official Full Name: X-box-binding protein 1 isoform XBP1(S)
• NCBI Official Synonym Full Names: X-box binding protein 1
• NCBI Official Symbol: Xbp1
• NCBI Official Synonym Symbols: TREB5; XBP-1; TREB-5; D11Ertd39e
• NCBI Protein Information: X-box-binding protein 1
• UniProt Protein Name: X-box-binding protein 1
• Protein Family: X-box-binding protein
• UniProt Gene Name: Xbp1
• UniProt Synonym Gene Names: TREB-5Curated

Uniprot Description

XBP1: a transcription factor essential for hepatocyte growth, the differentiation of plasma cells, immunoglobulin secretion, and the unfolded protein response (UPR). XBP1 mRNA is spliced by IRE1 during the UPR to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. Only the spliced form of XBP1 can activate the UPR efficiently. Activates UPR target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I. Up-regulated by ATF6 via direct binding to the ERSE in response to endoplasmic reticulum stress. Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7). Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature. Two human isoforms are produced by alternative splicing. Isoform 1 is also known as XBP-1U. Isoform 2, also known as XBP-1S, is produced by IRE1 in response to endoplasmic reticulum stress. IRE1 cleaves a 26-bp fragment causing a frameshift of the mRNA transcript.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 11 A1|11 3.61 cM

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; integral component of endoplasmic reticulum membrane; integral component of membrane; membrane; nucleus

Molecular Function: chromatin DNA binding; DNA binding; DNA binding transcription factor activity; histone deacetylase binding; identical protein binding; protease binding; protein heterodimerization activity; protein kinase binding; sequence-specific DNA binding; ubiquitin protein ligase binding

Biological Process: angiogenesis; apoptosis; autophagy; cell differentiation; cell growth; cellular response to glucose starvation; cellular response to insulin stimulus; cellular response to nutrient; cholesterol homeostasis; endoplasmic reticulum unfolded protein response; endothelial cell proliferation; epithelial cell maturation; exocrine pancreas development; fatty acid homeostasis; glucose homeostasis; lipid metabolic process; liver development; multicellular organism development; muscle organ development; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; neuron development; phosphoinositide 3-kinase cascade; positive regulation of angiogenesis; positive regulation of autophagy; positive regulation of B cell differentiation; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of fat cell differentiation; positive regulation of histone methylation; positive regulation of immunoglobulin production; positive regulation of immunoglobulin secretion; positive regulation of MHC class II biosynthetic process; positive regulation of protein phosphorylation; positive regulation of T cell differentiation; positive regulation of TOR signaling pathway; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription of target genes involved in unfolded protein response; protein destabilization; protein transport; regulation of autophagy; regulation of protein stability; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; response to drug; response to electrical stimulus; response to unfolded protein; serotonin secretion, neurotransmission; sterol homeostasis; transcription from RNA polymerase II promoter; transcription, DNA-dependent; transport; ubiquitin-dependent protein catabolic process; vascular endothelial growth factor receptor signaling pathway