Rat X-Box-Binding Protein 1 (XBP1) ELISA Kit | XBP1 elisa kit

Rat X-Box-Binding Protein 1 (XBP1) ELISA Kit

Cat. Num. AAA9711601

• Gene Names: Xbp1; HTF
• Reactivity: Rat
• Synonyms: X-Box-Binding Protein 1 (XBP1); Rat X-Box-Binding Protein 1 (XBP1) ELISA Kit; XBP1; TREB5; XBP2; XBP1 elisa kit

• Reactivity: Rat
• Specificity: Rat X-box-binding protein 1 (XBP1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat XBP1. No significant cross-reactivity or interference between Rat XBP1 and analogues was observed.
• Sequence Length: 267

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Rat X-box-binding protein 1 (XBP1) Standard Curve.)

Related Product Information for XBP1 elisa kit

Description: This Rat X-box-binding protein 1 (XBP1) ELISA Kit employs a two-site sandwich ELISA to quantitate XBP1 in samples. An antibody specific for XBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any XBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Chemokine (C motif) ligand (XCL1) is a small cytokine belonging to the XC chemokine family that is also known as lymphotactin. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. it shows little or no expression in colon and testis. Lymphotactin is chemotactic for CD4+ and CD8+ T cells but not for monocytes, and induces a rise in intracellular calcium in peripheral blood lymphocytes. Cellular sources for XCL1 include activated thymic and peripheral blood CD8 T cells. This chemokine attracts T cells. In humans, XCL1 is closely related to another chemokine called XCL2, whose gene is found at the same locus on chromosome 1. XCL1 induces it chemotactic function by binding to a chemokine receptor called XCR1.

NCBI and Uniprot Product Information

• NCBI GI #: 51948392
• NCBI GeneID: 289754
• NCBI Accession #: NP_001004210.1
• NCBI GenBank Nucleotide #: NP_001004210.1
• UniProt Accession #: Q9R1S4
• NCBI Official Full Name: X-box-binding protein 1 isoform XBP1(U)
• NCBI Official Synonym Full Names: X-box binding protein 1
• NCBI Official Symbol: Xbp1
• NCBI Official Synonym Symbols: HTF
• NCBI Protein Information: X-box-binding protein 1
• UniProt Protein Name: X-box-binding protein 1
• Protein Family: X-box-binding protein
• UniProt Gene Name: Xbp1
• UniProt Synonym Gene Names: HTF

NCBI Description

transcription factor; regulates MHC class II genes by binding to an X-box promoter element; agents that cause the unfolded protein response upregulate transcription [RGD, Feb 2006]

Uniprot Description

Functions as a transcription factor during endoplasmic reticulum (ER) stress by regulating the unfolded protein response (UPR). Required for cardiac myogenesis and hepatogenesis during embryonic development, and the development of secretory tissues such as exocrine pancreas and salivary gland. Involved in terminal differentiation of B lymphocytes to plasma cells and production of immunoglobulins. Modulates the cellular response to ER stress in a PIK3R-dependent manner. Binds to the cis-acting X box present in the promoter regions of major histocompatibility complex class II genes. Involved in VEGF-induced endothelial cell (EC) proliferation and retinal blood vessel formation during embryonic development but also for angiogenesis in adult tissues under ischemic conditions. Functions also as a major regulator of the UPR in obesity-induced insulin resistance and type 2 diabetes for the management of obesity and diabetes prevention.