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Typical Testing Data/Standard Curve (for reference only)

Human A kinase (PRKA) anchor protein 4 ELISA Kit | AKAP4 elisa kit

Human A-kinase anchor protein 4, AKAP4 ELISA Kit

Gene Names
AKAP4; HI; p82; CT99; FSC1; PRKA4; AKAP-4; AKAP82; AKAP 82; hAKAP82
Reactivity
Human
Synonyms
A kinase (PRKA) anchor protein 4; Human A-kinase anchor protein 4; AKAP4 ELISA Kit; Human A-kinase anchor protein 4 (AKAP4) ELISA kit; RP13-377G1__B.1; AKAP82; CT99; FSC1; HI; hAKAP82; p82; A-kinase anchor protein 4; A-kinase anchor protein 82 kDa; cancer/testis antigen 99; protein kinase A anchoring protein 4; testis-specific gene HI; AKAP4 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human AKAP4. No significant cross-reactivity or interference between human AKAP4 and analogues was observed.
Sequence Length
854
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
25 pg/ml-1600 pg/ml
Sensitivity
<6.25 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AKAP4 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AKAP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AKAP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AKAP4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AKAP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
94,477 Da
NCBI Official Full Name
A-kinase anchor protein 4 isoform 1
NCBI Official Synonym Full Names
A kinase (PRKA) anchor protein 4
NCBI Official Symbol
AKAP4
NCBI Official Synonym Symbols
HI; p82; CT99; FSC1; PRKA4; AKAP-4; AKAP82; AKAP 82; hAKAP82
NCBI Protein Information
A-kinase anchor protein 4; testis-specific gene HI; cancer/testis antigen 99; A-kinase anchor protein 82 kDa; major sperm fibrous sheath protein; protein kinase A anchoring protein 4; protein kinase A-anchoring protein 4
UniProt Protein Name
A-kinase anchor protein 4
Protein Family
UniProt Gene Name
AKAP4
UniProt Synonym Gene Names
AKAP82; AKAP-4; AKAP 82; hAKAP82; HI; PRKA4
UniProt Entry Name
AKAP4_HUMAN

NCBI Description

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

AKAP4: Major structural component of sperm fibrous sheath. Plays a role in sperm motility. Belongs to the AKAP110 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xp11.2

Cellular Component: cytoskeleton; perinuclear region of cytoplasm; cytoplasm; nucleus; Z disc; cAMP-dependent protein kinase complex

Molecular Function: protein kinase A binding

Biological Process: protein localization; sperm motility; cell projection organization and biogenesis; single fertilization; transmembrane receptor protein serine/threonine kinase signaling pathway; signal transduction; cell motility

Research Articles on AKAP4

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Product Notes

The Human AKAP4 akap4 (Catalog #AAA926560) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA926560 ELISA Kit recognizes Human AKAP4. It is sometimes possible for the material contained within the vial of "A kinase (PRKA) anchor protein 4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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