Mouse Vascular Endothelial Growth Factor B (VEGFB) ELISA Kit | VEGFB elisa kit

Mouse Vascular Endothelial Growth Factor B (VEGFB) Mini Samples ELISA Kit

Cat. Num. AAA2089509

• Gene Names: Vegfb; Vrf; VEGF-B
• Reactivity: Mouse
• Synonyms: Vascular Endothelial Growth Factor B (VEGFB); Mouse Vascular Endothelial Growth Factor B (VEGFB) Mini Samples ELISA Kit; VEGF-B; VEGFL; VRF; VEGF-Related Factor; VEGFB elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of VEGFA. No significant cross-reactivity or interference between VEGFA and analogues was observed.
• Sequence Length: 188

• Samples: Ovine Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: 5.7pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VEGFA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VEGFA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VEGFB elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of VEGFA in ovine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to VEGFA. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to VEGFA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VEGFA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of VEGFA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for VEGFB elisa kit

Cytokine; Tumor immunity; Infection immunity; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 297632418
• NCBI GeneID: 22340
• NCBI Accession #: NP_001172093.1
• NCBI GenBank Nucleotide #: NM_001185164.1
• UniProt Accession #: P49766; Q3UG04; Q5D0B1; Q64290
• Molecular Weight: 21,442 Da
• NCBI Official Full Name: vascular endothelial growth factor B isoform Vegf-b167
• NCBI Official Synonym Full Names: vascular endothelial growth factor B
• NCBI Official Symbol: Vegfb
• NCBI Official Synonym Symbols: Vrf; VEGF-B
• NCBI Protein Information: vascular endothelial growth factor B
• UniProt Protein Name: Vascular endothelial growth factor B
• Protein Family: Vascular endothelial growth factor
• UniProt Gene Name: Vegfb
• UniProt Synonym Gene Names: Vrf; VEGF-B; VRF

Uniprot Description

VEGFB: Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. Homodimer; disulfide-linked. Can also form heterodimer with VEGF. Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Cytokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19|19 A

Cellular Component: extracellular region; extracellular space; intracellular

Molecular Function: growth factor activity; protein heterodimerization activity; protein homodimerization activity; vascular endothelial growth factor receptor 1 binding

Biological Process: angiogenesis; cardiac muscle contraction; heart development; induction of positive chemotaxis; positive regulation of angiogenesis; positive regulation of endothelial cell proliferation; protein amino acid O-linked glycosylation; response to hypoxia; vascular endothelial growth factor receptor signaling pathway