Mouse Tyrosine-protein kinase BTK ELISA Kit | Btk elisa kit

Mouse Tyrosine-protein kinase BTK ELISA Kit

Cat. Num. AAA289589

• Gene Names: Btk; xid; AI528679
• Reactivity: Mouse
• Synonyms: Tyrosine-protein kinase BTK; Mouse Tyrosine-protein kinase BTK ELISA Kit; Agammaglobulinaemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase; Kinase EMB; Btk; Bpk; 2.7.10.2; Btk elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse Tyrosine-protein kinase BTK. No significant cross-reactivity or interference was observed.
• Sequence Length: 659

• Assay Type: Sandwich
• Detection Range: 0.156-10 ng/mL
• Sensitivity: 0.092 ng/mL
• Intra-Assay CV: <=8.8%
• Inter-Assay CV: <=11.0%
• Recovery: 99%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Btk elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of mouse Tyrosine-protein kinase BTK concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosine-protein kinase BTK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Tyrosine-protein kinase BTK and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Tyrosine-protein kinase BTK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Tyrosine-protein kinase BTK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 161016839
• NCBI GeneID: 12229
• NCBI Accession #: NP_038510.2
• NCBI GenBank Nucleotide #: NM_013482.2
• UniProt Accession #: P35991; Q61365
• Molecular Weight: 76,437 Da
• NCBI Official Full Name: tyrosine-protein kinase BTK
• NCBI Official Synonym Full Names: Bruton agammaglobulinemia tyrosine kinase
• NCBI Official Symbol: Btk
• NCBI Official Synonym Symbols: xid; AI528679
• NCBI Protein Information: tyrosine-protein kinase BTK
• UniProt Protein Name: Tyrosine-protein kinase BTK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Btk
• UniProt Synonym Gene Names: Bpk; ATK; BPK
• UniProt Entry Name: BTK_MOUSE

Uniprot Description

Btk: a tyrosine kinase of the Tec family. Plays a crucial role in B-cell ontogeny. Defects cause X-linked agammaglobulinemia, an immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. Truncated splice forms found in childhood leukemias may underlie radiation resistance of tumors through inhibition of apoptosis.

Protein type: Kinase, protein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; TK group; Tec family

Cellular Component: cytoplasm; cytoplasmic vesicle; cytosol; extrinsic to internal side of plasma membrane; intracellular membrane-bound organelle; lipid raft; mast cell granule; membrane; nucleus; perinuclear region of cytoplasm; plasma membrane

Molecular Function: ATP binding; identical protein binding; kinase activity; lipid binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; phosphatidylinositol-3,4,5-triphosphate binding; protein binding; protein kinase activity; protein-tyrosine kinase activity; transferase activity

Biological Process: adaptive immune response; apoptosis; cell maturation; histamine secretion by mast cell; I-kappaB kinase/NF-kappaB cascade; immune system process; innate immune response; negative regulation of cytokine production; peptidyl-tyrosine phosphorylation; phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of cell proliferation; regulation of transcription, DNA-dependent; response to organic substance; transcription, DNA-dependent; transmembrane receptor protein tyrosine kinase signaling pathway