Mouse Brutons tyrosine kinase Monoclonal Antibody | anti-Btk antibody

Brutons tyrosine kinase antibody

• Gene Names: Btk; xid; AI528679
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Brutons tyrosine kinase antibody was purified by ammonium sulfate precipitation
• Synonyms: Brutons tyrosine kinase; Monoclonal Antibody; Brutons tyrosine kinase antibody; Monoclonal Brutons tyrosine kinase; Anti-Brutons tyrosine kinase; BTK; Bruton's tyrosine kinase; Bruton agammaglobulinemia tyrosine kinase; agammaglobulinaemia tyrosine kinase; AGMX1; AT; atk; B-cell progenitor kinase; BOS_25575; bpk; bruton tyrosine kinase; dominant-negative kinase-deficient Brutons tyrosine kinase; imd1; kinase EMB; psctk1; RCJMB04_6h8; RP1-164F3.2; RP23-91G19.5; tyrosine-protein kinase BTK; Tyrosine-protein kinase BTK (Brutons tyrosine kinase) (Agammaglobulinaemia tyrosine kinase) (ATK) (B cell progenitor kinase) (BPK) (Kinase EMB); tyrosine-protein kinase BTK isoform (lacking exon 14); xid; XLA; X-linked immune deficiency; anti-Btk antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 kappa
• Clone Number: 1A1
• Purity/Purification: Brutons tyrosine kinase antibody was purified by ammonium sulfate precipitation
• Form/Format: Supplied in liquid form in HEPES buffer with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol
• Sequence Length: 659

• Applicable Applications for anti-Btk antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: WB: 1:5000
• Biological Significance: Btk (Brutons tyrosine kinase) is a member of the Tec family of protein tyrosine kinases (PTKs) and plays a modulatory role in many cellular processes, such as proliferation, development, differentiation, survival, and apoptosis. The Tec kinases are the second largest family of non-receptor tyrosine kinases and include Tec, Btk, Bmx, Itk, and TXK/Rlk. Mutations of Btk gene cause a primary immunodeficiency disease in humans, X-linked agammaglobulinemia (XLA) which is characterized by a lack of circulating B lymphocytes and an absence of immunoglobulins as a result of defects in B cell maturation and function. Btk is found in all hematopoietic cells, with the exception of T lymphocytes and plasma cells. Btk contains amino-terminal PH (pleckstrin homology) domain which binds phosphatidylinositol (3,4,5)-trisphosphate (PIP3) helping membrane translocation upon PI3 kinase activation.
• Cross-Reactivity: Human
• Immunogen: Brutons tyrosine kinase antibody was raised in Mouse using a recombinant human protein purified from E Coli (His/ABD-Btk) as the immunogen
• Preparation and Storage: Aliquot and store for 1 year at -20 degree C, avoid freeze/thaw cycles

Western Blot (WB)

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Related Product Information for anti-Btk antibody

Mouse monoclonal Brutons tyrosine kinase antibody

Product Categories/Family for anti-Btk antibody

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 31419802
• NCBI GeneID: 12229
• Molecular Weight: 76,437 Da
• NCBI Official Full Name: Btk protein
• NCBI Official Synonym Full Names: Bruton agammaglobulinemia tyrosine kinase
• NCBI Official Symbol: Btk
• NCBI Official Synonym Symbols: xid; AI528679
• NCBI Protein Information: tyrosine-protein kinase BTK
• UniProt Protein Name: Tyrosine-protein kinase BTK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Btk
• UniProt Synonym Gene Names: Bpk; ATK; BPK
• UniProt Entry Name: BTK_MOUSE

Uniprot Description

Btk: a tyrosine kinase of the Tec family. Plays a crucial role in B-cell ontogeny. Defects cause X-linked agammaglobulinemia, an immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. Truncated splice forms found in childhood leukemias may underlie radiation resistance of tumors through inhibition of apoptosis.

Protein type: Kinase, protein; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; EC 2.7.10.2; TK group; Tec family

Cellular Component: extrinsic to internal side of plasma membrane; mast cell granule; intracellular membrane-bound organelle; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytoplasmic vesicle; intracellular; nucleus; cytosol; lipid raft

Molecular Function: transferase activity; identical protein binding; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; metal ion binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; kinase activity; lipid binding; ATP binding; protein kinase activity

Biological Process: I-kappaB kinase/NF-kappaB cascade; adaptive immune response; peptidyl-tyrosine phosphorylation; transcription, DNA-dependent; immune system process; apoptosis; protein amino acid autophosphorylation; cell maturation; protein amino acid phosphorylation; regulation of cell proliferation; response to organic substance; B cell receptor signaling pathway; histamine secretion by mast cell; regulation of transcription, DNA-dependent; innate immune response; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway