Human Tyrosine-protein kinase BTK ELISA Kit | BTK elisa kit

Human Tyrosine-protein kinase BTK ELISA Kit

Cat. Num. AAA760610

• Gene Names: Btk; xid; AI528679
• Reactivity: Human
• Synonyms: Tyrosine-protein kinase BTK; Human Tyrosine-protein kinase BTK ELISA Kit; BTK/Tyrosine-protein kinase BTK/Bruton tyrosine kinase/Agammaglobulinaemia tyrosine kinase/ATK/B-cell progenitor kinase/BPK/AT/XLA/IMD1/AGMX1/PSCTK1/dominant-negative kinase-deficient Brutons tyrosine kinase/Bruton agammaglobulinemia tyrosine kinase; BTK elisa kit

• Reactivity: Human
• Sequence Length: 659

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for BTK elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human BTK antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human BTK antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human BTK amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman BTK can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 161016839
• NCBI GeneID: 12229
• NCBI Accession #: NP_038510.2
• NCBI GenBank Nucleotide #: NM_013482.2
• Molecular Weight: 76,437 Da
• NCBI Official Full Name: tyrosine-protein kinase BTK
• NCBI Official Synonym Full Names: Bruton agammaglobulinemia tyrosine kinase
• NCBI Official Symbol: Btk
• NCBI Official Synonym Symbols: xid; AI528679
• NCBI Protein Information: tyrosine-protein kinase BTK
• UniProt Protein Name: Tyrosine-protein kinase BTK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Btk
• UniProt Synonym Gene Names: Bpk; ATK; BPK
• UniProt Entry Name: BTK_MOUSE

Uniprot Description

Btk: a tyrosine kinase of the Tec family. Plays a crucial role in B-cell ontogeny. Defects cause X-linked agammaglobulinemia, an immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. Truncated splice forms found in childhood leukemias may underlie radiation resistance of tumors through inhibition of apoptosis.

Protein type: EC 2.7.10.2; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Kinase, protein; TK group; Tec family

Cellular Component: cytoplasm; cytoplasmic vesicle; cytosol; extrinsic to internal side of plasma membrane; intracellular membrane-bound organelle; lipid raft; mast cell granule; membrane; nucleus; perinuclear region of cytoplasm; plasma membrane

Molecular Function: ATP binding; identical protein binding; kinase activity; lipid binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; phosphatidylinositol-3,4,5-triphosphate binding; protein binding; protein kinase activity; protein-tyrosine kinase activity; transferase activity

Biological Process: adaptive immune response; apoptosis; cell maturation; histamine secretion by mast cell; I-kappaB kinase/NF-kappaB cascade; immune system process; innate immune response; negative regulation of cytokine production; peptidyl-tyrosine phosphorylation; phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of cell proliferation; regulation of transcription, DNA-dependent; response to organic substance; transcription, DNA-dependent; transmembrane receptor protein tyrosine kinase signaling pathway