Rabbit anti-Human Rad9 Polyclonal Antibody | anti-Rad9 antibody

Rad9, Phosphorylated (Ser328) (RAD9A, Cell Cycle Checkpoint Control Protein RAD9A, hRAD9, DNA Repair Exonuclease Rad9 Homolog A) (FITC)

Cat. Num. AAA6496477

• Gene Names: RAD9A; RAD9
• Reactivity: Human
• Applications: Western Blot, Dot Blot, Immunohistochemistry
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Rad9; Polyclonal Antibody; Phosphorylated (Ser328) (RAD9A; Cell Cycle Checkpoint Control Protein RAD9A; hRAD9; DNA Repair Exonuclease Rad9 Homolog A) (FITC); anti-Rad9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Rad9 when phosphorylated at Ser328.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-Rad9 antibody: FLISA, Western Blot (WB), Dot Blot (DB), Immunohistochemistry (IHC)
• Application Notes: FLISA: 1:1,000; Dot Blot: 1:500; IHC: 1:50-1:100; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser328 of human Rad9 (KLH).
• Conjugate: FITC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-Phospho-Rad9-S328 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Western Blot (WB)

(The anti-Phospho-Rad9-S328 Pab is used in Western blot to detect Phospho-Rad9-S328 in HL60 (left) and Y79 (right) tissue lysates.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-Rad9 antibody

Antibodies; DNA Replication; Repair

References

1. Meng, Y. et al. BMC Cell Biol. 10-96(2009). 2. Maniwa, Y., et al., Cancer 103(1):126-132 (2005). 3. Wang, W., et al., Proc. Natl. Acad. Sci. USA 101(48):16762-16767 (2004). 4. Lindsey-Boltz, L.A., et al., (er) Nucleic Acids Res. 32(15):4524-4530 (2004). 5. Toueille, M., et al., (er) Nucleic Acids Res. 32(11):3316-3324 (2004). 6.Loegering, D., et al., J. Biol. Chem. 279(18):18641-18647 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 4759022
• NCBI GeneID: 5883
• NCBI Accession #: NP_004575
• NCBI GenBank Nucleotide #: NM_004584
• UniProt Accession #: Q99638; Q6FI29; Q96C41; B2RCZ8
• Molecular Weight: 42,547 Da
• NCBI Official Full Name: cell cycle checkpoint control protein RAD9A isoform 1
• NCBI Official Synonym Full Names: RAD9 homolog A (S. pombe)
• NCBI Official Symbol: RAD9A
• NCBI Official Synonym Symbols: RAD9
• NCBI Protein Information: cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A; hRAD9
• UniProt Protein Name: Cell cycle checkpoint control protein RAD9A
• Protein Family: RAD9, HUS1, RAD1-interacting nuclear orphan protein
• UniProt Gene Name: RAD9A
• UniProt Synonym Gene Names: hRAD9
• UniProt Entry Name: RAD9A_HUMAN

Uniprot Description

Rad9: a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. Possesses a 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. Forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for Rad9.

Protein type: EC 3.1.11.2; DNA replication; Deoxyribonuclease; Apoptosis; Cell cycle regulation

Chromosomal Location of Human Ortholog: 11q13.1-q13.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; histone deacetylase binding; exodeoxyribonuclease III activity; 3'-5' exonuclease activity; SH3 domain binding; protein kinase binding

Biological Process: intra-S DNA damage checkpoint; DNA damage checkpoint; DNA replication checkpoint; DNA replication; DNA repair; DNA catabolic process, exonucleolytic; response to DNA damage stimulus