Bovine S100 Calcium Binding Protein A7 ELISA Kit | S100A7 elisa kit

Bovine S100 Calcium Binding Protein A7 ELISA Kit

Cat. Num. AAA740592

• Reactivity: Bovine
• Synonyms: S100 Calcium Binding Protein A7; Bovine S100 Calcium Binding Protein A7 ELISA Kit; S100A7 elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This S100A7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine S100A7. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S100A7 elisa kit

Principle of the Assay: S100A7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-S100A7 antibody and an S100A7-HRP conjugate. The assay sample and buffer are incubated together with S100A7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the S100A7 concentration since S100A7 from samples and S100A7-HRP conjugate compete for the anti-S100A7 antibody binding site. Since the number of sites is limited, as more sites are occupied by S100A7 from the sample, fewer sites are left to bind S100A7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100A7 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for S100A7 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 49457324
• UniProt Accession #: P31151; Q5SY67; Q6FGE3; Q9H1E2
• Molecular Weight: 11,471 Da
• NCBI Official Full Name: S100A7
• UniProt Protein Name: Protein S100-A7
• Protein Family: Protein
• UniProt Gene Name: S100A7
• UniProt Synonym Gene Names: PSOR1; S100A7C
• UniProt Entry Name: S10A7_HUMAN

Uniprot Description

S100A7: is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein differs from the other S100 proteins of known structure in its lack of calcium binding ability in one EF-hand at the N-terminus. This protein is markedly over-expressed in the skin lesions of psoriatic patients, but is excluded as a candidate gene for familial psoriasis susceptibility. The exact function of this protein is not known. [provided by RefSeq, Jul 2008]

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: focal adhesion; endoplasmic reticulum; cytoplasm; extracellular region; cytosol; nucleus

Molecular Function: protein binding; RAGE receptor binding; zinc ion binding; calcium ion binding

Biological Process: keratinocyte differentiation; sequestering of metal ion; response to reactive oxygen species; epidermis development; innate immune response; response to lipopolysaccharide; angiogenesis; defense response to Gram-negative bacterium