Human Tubulin Alpha 4A (TUBa4A) RTU ELISA Kit | TUBa4A rtu elisa kit

Human Tubulin Alpha 4A (TUBa4A) ELISA Kit

Cat. Num. AAA4503687

• Gene Names: TUBA4A; ALS22; TUBA1; H2-ALPHA
• Reactivity: Human
• Synonyms: Tubulin Alpha 4A (TUBa4A); Human Tubulin Alpha 4A (TUBa4A) ELISA Kit; TUBA1; Testis Specific Tubulin Alpha 1; Alpha-tubulin 1; Tubulin alpha-1 chain; Tubulin H2-alpha; TUBa4A rtu elisa kit

• Reactivity: Human
• Sequence Length: 433

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.058ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TUBa4A rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TUBa4A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TUBa4A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TUBa4A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TUBa4A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 514052659
• NCBI GeneID: 7277
• NCBI Accession #: NP_001265481.1
• NCBI GenBank Nucleotide #: NM_001278552.1
• UniProt Accession #: P68366; P05215; A8MUB1; B3KNQ6
• Molecular Weight: 48,329 Da
• NCBI Official Full Name: tubulin alpha-4A chain isoform 2
• NCBI Official Synonym Full Names: tubulin alpha 4a
• NCBI Official Symbol: TUBA4A
• NCBI Official Synonym Symbols: ALS22; TUBA1; H2-ALPHA
• NCBI Protein Information: tubulin alpha-4A chain
• UniProt Protein Name: Tubulin alpha-4A chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBA4A
• UniProt Synonym Gene Names: TUBA1

NCBI Description

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.