Typical Testing Data/Standard Curve (for reference only)
(Mouse TPOR/MPL PicoKine ELISA Kit standard curve )
Mouse TPOR/MPL ELISA Kit | MPL elisa kit
Mouse TPOR/MPL PicoKine ELISA Kit
Gene Names
Mpl; CD110; TPO-R; c-mpl; hlb219
Reactivity
Mouse
Synonyms
TPOR/MPL; Mouse TPOR/MPL PicoKine ELISA Kit; C MPL; CD110; mpl; MPLV; THCYT2; Thrombopoietin receptor; TPOR; TPO-R; TPO R; Q08351; MPL proto-oncogene; thrombopoietin receptor; MPL elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse TPOR/MPL
Sequence Length
633
Samples
Cell culture supernates, serum and plasma(heparin, EDTA).
Assay Type
Sandwich
Detection Range
156pg/ml-10000pg/ml
Sensitivity
<15pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
Typical Testing Data/Standard Curve (for reference only)
(Mouse TPOR/MPL PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse TPOR/MPL PicoKine ELISA Kit standard curve )
Related Product Information for MPL elisa kit
Principle of the Assay: Mouse TPOR/MPL ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TPOR/MPL has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: Q26-W482) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TPOR/MPL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse TPOR/MPL amount of sample captured in plate.
Background/Introduction: The thrombopoietin receptor (TPOR), also known as CD110, is a protein that in humans is encoded by the MPL (myeloproliferative leukemia virus) oncogene. The human MPL gene is mapped to chromosome 1p34. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.
Background/Introduction: The thrombopoietin receptor (TPOR), also known as CD110, is a protein that in humans is encoded by the MPL (myeloproliferative leukemia virus) oncogene. The human MPL gene is mapped to chromosome 1p34. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69,788 Da
NCBI Official Full Name
thrombopoietin receptor isoform 1
NCBI Official Synonym Full Names
myeloproliferative leukemia virus oncogene
NCBI Official Symbol
Mpl
NCBI Official Synonym Symbols
CD110; TPO-R; c-mpl; hlb219
NCBI Protein Information
thrombopoietin receptor
UniProt Protein Name
Thrombopoietin receptor
Protein Family
UniProt Gene Name
Mpl
UniProt Synonym Gene Names
Tpor; TPO-R
UniProt Entry Name
TPOR_MOUSE
Uniprot Description
MPL: a hematopoietic receptor for thrombopoietin. Thrombopoietin is the major regulator of megakaryocytopoiesis and platelet formation. Dimerizes upon binding of thrombopoietin, leading to its phosphorylation and activation of JAKs and STATs. May represent a regulatory molecule specific for TPO-R-dependent immune responses. Two splice-variant isoforms have been described.
Protein type: Membrane protein, integral; Receptor, cytokine
Cellular Component: cell soma; cell surface; Golgi apparatus; neuron projection; plasma membrane
Biological Process: homeostasis of number of cells; negative regulation of apoptosis; platelet formation; positive regulation of lymphocyte proliferation; regulation of chemokine production
Research Articles on MPL
Similar Products
Product Notes
The Mouse MPL mpl (Catalog #AAA178526) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA178526 ELISA Kit recognizes Mouse MPL. It is sometimes possible for the material contained within the vial of "TPOR/MPL, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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