Typical Testing Data/Standard Curve (for reference only)
Mouse CD110 ELISA Kit | MPL elisa kit
Mouse CD110 ELISA Kit
Gene Names
Mpl; CD110; TPO-R; c-mpl; hlb219
Reactivity
Mouse
Synonyms
CD110; Mouse CD110 ELISA Kit; TPOR; Thrombopoietin receptor; TPO-R; Myeloproliferative leukemia protein; Proto-oncogene c-Mpl; MPL elisa kit
Reactivity
Mouse
Specificity
The Mouse CD110 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CD110 proteins within the range of 156 pg/ml - 10000 pg/ml.
Sequence Length
633
Samples
Cell Culture Supernates, Serum, Plasma
Sensitivity
<15 pg/ml
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MPL elisa kit
Principle of the Assay: The Mouse CD110 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD110 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse CD110 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD110 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD110 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD110 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background: The thrombopoietin receptor (TPOR), also known as CD110, is a protein that in humans is encoded by the MPL (myeloproliferative leukemia virus) oncogene. The human MPL gene is mapped to chromosome 1p34. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.
Background: The thrombopoietin receptor (TPOR), also known as CD110, is a protein that in humans is encoded by the MPL (myeloproliferative leukemia virus) oncogene. The human MPL gene is mapped to chromosome 1p34. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69,788 Da
NCBI Official Full Name
thrombopoietin receptor isoform 1
NCBI Official Synonym Full Names
myeloproliferative leukemia virus oncogene
NCBI Official Symbol
Mpl
NCBI Official Synonym Symbols
CD110; TPO-R; c-mpl; hlb219
NCBI Protein Information
thrombopoietin receptor
UniProt Protein Name
Thrombopoietin receptor
Protein Family
UniProt Gene Name
Mpl
UniProt Synonym Gene Names
Tpor; TPO-R
UniProt Entry Name
TPOR_MOUSE
Uniprot Description
MPL: a hematopoietic receptor for thrombopoietin. Thrombopoietin is the major regulator of megakaryocytopoiesis and platelet formation. Dimerizes upon binding of thrombopoietin, leading to its phosphorylation and activation of JAKs and STATs. May represent a regulatory molecule specific for TPO-R-dependent immune responses. Two splice-variant isoforms have been described.
Protein type: Membrane protein, integral; Receptor, cytokine
Cellular Component: cell soma; cell surface; Golgi apparatus; neuron projection; plasma membrane
Biological Process: homeostasis of number of cells; negative regulation of apoptosis; neutrophil homeostasis; platelet formation; positive regulation of lymphocyte proliferation; regulation of chemokine production
Research Articles on MPL
Similar Products
Product Notes
The Mouse MPL mpl (Catalog #AAA8248451) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8248451 ELISA Kit recognizes Mouse MPL. It is sometimes possible for the material contained within the vial of "CD110, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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