Mouse Toll Like Receptor Adaptor Molecule 1 (TICAM1) ELISA Kit | TICAM1 elisa kit

Mouse Toll Like Receptor Adaptor Molecule 1 (TICAM1) ELISA Kit

Cat. Num. AAA2706295

• Gene Names: TICAM1; TRIF; IIAE6; MyD88-3; PRVTIRB; TICAM-1
• Reactivity: Mouse
• Synonyms: Toll Like Receptor Adaptor Molecule 1 (TICAM1); Mouse Toll Like Receptor Adaptor Molecule 1 (TICAM1) ELISA Kit; TRIF; PRVTIRB; Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta; Proline-rich; vinculin and TIR domain-containing protein B; TICAM1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Toll Like Receptor Adaptor Molecule 1 (TICAM1).; No significant cross-reactivity or interference between Toll Like Receptor Adaptor Molecule 1 (TICAM1) and analogues was observed.
• Sequence Length: 712

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 62.5-4000pg/mL
• Sensitivity: < 21.7pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Toll Like Receptor Adaptor Molecule 1 (TICAM1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Toll Like Receptor Adaptor Molecule 1 (TICAM1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TICAM1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor Adaptor Molecule 1 (TICAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Toll Like Receptor Adaptor Molecule 1 (TICAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Toll Like Receptor Adaptor Molecule 1 (TICAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Toll Like Receptor Adaptor Molecule 1 (TICAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 223460864
• NCBI GeneID: 148022
• UniProt Accession #: Q8IUC6; O75532; Q86XP8; Q96GA0; B3Y691
• Molecular Weight: 76,422 Da
• NCBI Official Full Name: Toll-like receptor adaptor molecule 1
• NCBI Official Synonym Full Names: toll like receptor adaptor molecule 1
• NCBI Official Symbol: TICAM1
• NCBI Official Synonym Symbols: TRIF; IIAE6; MyD88-3; PRVTIRB; TICAM-1
• NCBI Protein Information: TIR domain-containing adapter molecule 1
• UniProt Protein Name: TIR domain-containing adapter molecule 1
• Protein Family: TIR domain-containing adapter molecule
• UniProt Gene Name: TICAM1
• UniProt Synonym Gene Names: PRVTIRB; TRIF; TICAM-1; MyD88-3; TIR domain-containing adapter protein inducing IFN-beta

NCBI Description

This gene encodes an adaptor protein containing a Toll/interleukin-1 receptor (TIR) homology domain, which is an intracellular signaling domain that mediates protein-protein interactions between the Toll-like receptors (TLRs) and signal-transduction components. This protein is involved in native immunity against invading pathogens. It specifically interacts with toll-like receptor 3, but not with other TLRs, and this association mediates dsRNA induction of interferon-beta through activation of nuclear factor kappa-B, during an antiviral immune response. [provided by RefSeq, Jan 2012]

Uniprot Description

Involved in innate immunity against invading pathogens. Adapter used by TLR3 and TLR4 (through TICAM2) to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively.